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Product Name Anti-DZIP3/PE-Cy7 Chinese Name PE-Cy7标记的E3Ubiquitin蛋白连接酶dzip3抗体 Alias DAZ-interacting protein 3; E3 ubiquitin-protein ligase DZIP3; hRUL138; KIAA0675; RNA-binding ubiquitin ligase of 138 kDa; DZIP3_HUMAN. Research Area Cell biology Epigenetics Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Recombinant Fragment) Applications ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 139kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DZIP3 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). DZIP3 (DAZ interacting protein 3, zinc finger), also known as UURF2 or hRUL138, is a 1,208 amino acid protein that localizes to the cytoplasm and contains one RING-type zinc finger. Expressed in a variety of tissues with highest expression in heart, skeletal muscle and kidney, DZIP3 functions as an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme, thereby playing a role in signaling events throughout the cell. Multiple isoforms of DZIP3 exist due to alternative splicing events.
Function:
E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.
Subunit:
Interacts with DAZ proteins.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Widely expressed at low level. Highlyexpressed in skeletal muscle, kidney and heart. Expressed at lowlevel in placenta, lung, brain, liver and pancreas.
Similarity:
Contains 1 RING-type zinc finger.
Database links:
UniProtKB/Swiss-Prot: Q86Y13.2
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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