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Product Name Anti-Calpain 1/PE-Cy5.5 Chinese Name PE-Cy5.5标记的钙蛋白酶1抗体 Alias Calcium activated neutral proteinase 1; Calpain1; Calpain-1; Calpain 1 catalytic subunit; Calpain large polypeptide L1; Calpain mu type; CANP 1; CANP; CANP1; CANPL1; CAPN 1; CAPN1; Cell proliferation inducing gene 30 protein; Cell proliferation inducing protein 30; Micromolar calpain; muCANP; muCL; Ca2 activated neutral protease; Calcium activated neutral proteinase small subunit; Calcium dependent protease small subunit 1; Calcium-activated neutral proteinase 1; Calpain 1 (mu/I) large subunit; Calpain 1 catalytic subunit; Calpain 1 large subunit; Calpain Large Polypeptide L1; Calpain Large Polypeptide L1; Calpain mu type; Calpain mu-type; Calpain regulatory subunit; Calpain small subunit 1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain1; CAN1_HUMAN. Research Area Signal transduction Apoptosis Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Pig, Cow, Sheep, ) Applications Flow-Cyt=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 78kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse Calpain 1 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Function:
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Subunit:
Forms a heterodimer with a small (regulatory) subunit (CAPNS1).
Subcellular Location:
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.
Similarity:
Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains.
Database links:Entrez Gene: 12333 Mouse
Entrez Gene: 823 Human
Omim: 114220 Human
SwissProt: P07384 Human
SwissProt: O35350 Mouse
Unigene: 502842 Human
Unigene: 6221 Mouse
Unigene: 6037 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
钙蛋白酶(calpain)是一族钙离子依赖性的、水解半胱氨酸的蛋白酶,广泛存在于全身各脏器组织中。钙蛋白酶参与不同细胞过程,包括Cytoskeleton和The cell membrane附着的构型重建、不同的Signal transduction途径和Apoptosis等.
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