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Product Name Anti-laminin/PE-Cy5.5 Chinese Name PE-Cy5.5标记的层粘连蛋白抗体 Alias LAMA 1; LAMA; LAMA1; LAMB1; Laminin A chain; Laminin alpha 1; Laminin alpha 1 chain; Laminin B1 chain; Laminin subunit beta 1. Research Area Tumour Cell biology immunology Neurobiology Signal transduction Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Cow, ) Applications IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 198kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human laminin Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
laminins are the major noncollagenous components of basement membranes that mediate cell adhesion, growth migration, and differentiation; the laminin-type epidermal growth factor-like module occurs in tandem arrays; the domain contains 4 disulfide bonds (loops a-d) the first three resemble epidermal growth factor (EGF); the number of copies of this domain in the different forms of laminins is highly variable ranging from 3 up to 22 copies.
Function:
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Subcellular Location:
Secreted > extracellular space > extracellular matrix > basement membrane.
Tissue Specificity:
Broadly expressed in: skin, heart, lung, and the reproductive tracts.
Similarity:
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
Database links:Entrez Gene: 10319 Human
Entrez Gene: 284217 Human
Entrez Gene: 3908 Human
Entrez Gene: 3912 Human
Entrez Gene: 3913 Human
Entrez Gene: 3915 Human
Entrez Gene: 3918 Human
Entrez Gene: 16772 Mouse
Omim: 150320 Human
SwissProt: P07942 Human
SwissProt: P24043 Human
SwissProt: P25391 Human
SwissProt: P55268 Human
SwissProt: Q9Y6N6 Human
SwissProt: P19137 Mouse
SwissProt: Q9R0B6 Mouse
Unigene: 201805 Human
Unigene: 270364 Human
Unigene: 302362 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
层粘连蛋白主要存在于基膜(basal lamina)结构中,是基膜所特有的非胶原glycoprotein。
层粘连蛋白在细胞发育过程中刺激细胞粘着、细胞运动。LN能够刺激胚胎中神经轴的生长,并促进成年动物的神经损伤后重生长和再生。如同纤粘连蛋白,细胞外的LN能够影响细胞的生长、迁移和分化。LN在原生殖细胞的迁移中起关键作用。
层粘连蛋白是细胞外基底膜的主要组成部分,分布于基底膜和Extracellular matrix内、成纤维细胞、endothelial cells和平滑肌细胞分泌层粘连蛋白。Tumour的浸润和转移与其遭到破坏相关。该抗体可用于标记基底膜,有助于观察Tumour周围基底膜的分布及其改变,间接提示该Tumour的分化程度和浸润情况。
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