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Product Name Anti-Nestin/PE-Cy3 Chinese Name PE-Cy3标记的巢蛋白/神经上皮Stem cells蛋白抗体 Alias ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin. Research Area Tumour Cardiovascular Developmental biology Neurobiology Stem cells Cell type markers TumourCell biologyMaker Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, Applications Flow-Cyt=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 209kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from rat Nestin Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
cytoplasm
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
Database links:
Entrez Gene: 10763 Human
Entrez Gene: 18008 Mouse
Omim: 600915 Human
SwissProt: P48681 Human
SwissProt: Q6P5H2 Mouse
Unigene: 527971 Human
Unigene: 331129 Mouse
Unigene: 9701 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
神经Stem cellsMaker
在动物体早期发育过程中,中枢神经系统发育尤为重要 。Nestin是一种中等纤维蛋白,它在哺乳动物神经前体细胞中表达较高,目前已被广泛用于神经前体细胞的重要标志分子。研究Nestin的表达调控规律,将有助于人们认识神经前体细胞的生物特性。此抗体还表达中枢神经系统Stem cells及神经epithelial cells。
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