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Product Name Anti-AHA3/PE-Cy3 Chinese Name PE-Cy3标记的AHA3抗体(拟南芥) Alias ATPase/hydrogen-exporting ATPase, phosphorylative mechanism; member of Plasma membrane H+-ATPase family; PMA3_ARATH. Product Type Botany抗体 Research Area Cell biology immunology transcriptional regulatory factor Botany Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Arabidopsis Thaliana, ) Applications IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 104kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Arabidopsis thaliana (thale cress) AHA3 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail Function:
The plasma membrane H(+) ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
Subunit:
Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H(+)-ATPase.
Subcellular Location:
Cell membrane; Multi-pass membrane proteinc(Probable).
Tissue Specificity:
Found predominantly in phloem cells of leaves, stems, roots and flowers.
Post-translational modifications:
Phosphorylation level varies significantly during early response to bacterial elicitor (e.g. fusicoccin FC).
Similarity:
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.
Database links:Entrez Gene: 835841 Arabidopsis thaliana
SwissProt: P20431 Arabidopsis thaliana
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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