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Product Name Anti-phospho-SPHK2 (Thr614)/HRP Chinese Name 辣根过氧化物酶标记的磷酸化鞘氨醇激酶2抗体 Alias SPHK2 (phospho T614); p-SPHK2 (phospho T614); SK 2; Sphingosine kinase 2; Sphingosine kinase type 2; SPHK2; SPHK2_HUMAN; SPK 2. Product Type Phosphorylated anti Research Area Tumour Cell biology Neurobiology Signal transduction Kinases and Phosphatases G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 72kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human SPHK2 around the phosphorylation site of Thr614 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Function:
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
Subcellular Location:
Cytoplasm. Membrane and Lysosome membrane.
Similarity:
Contains 1 DAGKc domain.
Database links:Entrez Gene: 56848 Human
Entrez Gene: 56632 Mouse
Omim: 607092 Human
SwissProt: Q9NRA0 Human
SwissProt: Q9JIA7 Mouse
Unigene: 528006 Human
Unigene: 24222 Mouse
Unigene: 41053 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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