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Product Name Anti-AES/HRP Chinese Name 辣根过氧化物酶标记的gp130Binding proteinGAM抗体 Alias Aes 1; Amino-terminal enhancer of split; Aes 2; aes; AES_HUMAN; Aes1; Aes2; Amino Enhancer of Split; Amino terminal enhancer of split; Amino-terminal enhancer of split; Esp 1; Esp1; Gp130 associated protein GAM; Gp130-associated protein GAM; GRG 5; GRG; GRG protein; GRG5; Protein ESP 1; Protein ESP1; Protein GRG; TLE 5; TLE5. Research Area Cell biology Signal transduction Stem cells transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 22kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TLE5/Amino-terminal enhancer of split Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
TLE5 is a 197 amino acid nuclear protein that belongs to the TLE family. Expressed predominately in fetal brain, liver, lung, heart and kidney and in adult muscle, TLE5 functions as either a homooligomer or a heterooligomer with other TLE family members and, through this association, dominantly represses the expression of TLE genes. In addition, TLE5 can repress NFkB-regulated gene expression and is thought to play an important role in initiating and maintaining cell differentiation events. Two isoforms of TLE5 exist due to alternative splicing events.
Function:
Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state.
Subunit:
Homooligomer and heterooligomer with other family members. Binds TCF7 (By similarity). Binds the NF-kappa-B subunit RELA. Interacts with PHF12.
Subcellular Location:
Nucleus.
Tissue Specificity:
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
Post-translational modifications:
Ubiquitinated by XIAP/BIRC4.
Similarity:
Belongs to the WD repeat Groucho/TLE family.
Database links:Entrez Gene: 166 Human
Entrez Gene: 14797 Mouse
Omim: 600188 Human
SwissProt: Q08117 Human
SwissProt: P63002 Mouse
Unigene: 515053 Human
Unigene: 180013 Mouse
Unigene: 11495 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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