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Product Name Anti-Engrailed 1/HRP Chinese Name 辣根过氧化物酶标记的同源盒蛋白转录因子EN1抗体 Alias EN1 / Engrailed 1; En 1; EN1; EN-1; Engrailed 1; Engrailed; Engrailed homeobox 1; Engrailed homolog 1; HME1_HUMAN; Homeobox protein en-1; Homeobox protein engrailed 1; Homeobox protein engrailed-1; Hu-En-1; Mo-en.1. Research Area Cell biology Developmental biology Neurobiology Signal transduction transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) Applications ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 40kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human EN1/Engrailed 1 (351-420aa) Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
The engrailed-1 gene, EN1, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homo-logs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expres-sion of a-synuclein, a gene that is genetically linked to Parkinson’s disease.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the engrailed homeobox family.
Contains 1 homeobox DNA-binding domain.
Database links:Entrez Gene: 2019 Human
Entrez Gene: 13798 Mouse
Omim: 131290 Human
SwissProt: Q05925 Human
SwissProt: P09065 Mouse
Unigene: 271977 Human
Unigene: 2657 Mouse
Unigene: 162354 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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