Product Name	Anti-ACA11/HRP
Chinese Name	辣根过氧化物酶标记的拟南芥ACA11抗体
Alias	calcium-transporting ATPase; Putative calcium-transporting ATPase 11, plasma membrane-type; Ca(2+)-ATPase isoform 11; autoinhibited Ca2+-ATPase 11(Arabidopsis thaliana); ACA11_ARATH.
Product Type	Botany抗体
Research Area	Botany
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	(predicted: Arabidopsis Thaliana, )
Applications	WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Molecular weight	112 kDa
Form	Lyophilized or Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from Arabidopsis thaliana ACA11
Lsotype	IgG
Purification	affinity purified by Protein A
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Product Detail	background: In plant cells, the vacuole functions as a major calcium store. Although a calmodulin-regulated Ca2+-ATPase (ACA4) is known to be present in prevacuolar compartments, the presence of an ACA-type Ca2+-ATPase in the mature vacuole of a plant cell has not been verified. Here we provide evidence that ACA11 localizes to the vacuole membrane. ACA11 tagged with GFP was expressed in stable transgenic plants, and visualized in root cells and protoplasts by confocal microscopy. A Ca2+-ATPase function for ACA11 was confirmed by complementation of yeast mutants. Function: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles (By similarity). Subcellular Location: Membrane; Multi-pass membrane protein. Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. Database links: Entrez Gene: 824900 ARATH SwissProt: Q9M2L4 ARATH Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 拟南芥

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