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Product Name Anti-CYB5R2/Gold Chinese Name 胶体金标记的细胞色素B5还原酶2抗体 Alias b5R.2; Cyb5r2; Cytochrome b5 reductase 2; Cytochrome b5 reductase b5R.2; NADH cytochrome b5 reductase 2; NADH-cytochrome b5 reductase 2; NB5R2_HUMAN. Research Area Tumour Cell biology Neurobiology Signal transduction The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Cow, Horse, Sheep, ) Applications IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 31kDa Form Lyophilized or Liquid Concentration 0.4mg/ml immunogen KLH conjugated synthetic peptide derived from human CYB5R2 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. Storage Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. Product Detail background:
NADH-cytochrome b5 reductases participate in a variety of physiological processes including biosynthesis of cholesterol, methemoglobin reduction of erythrocytes, elongation of fatty acids and metabolism of drugs. CYB5R2 (cytochrome b5 reductase 2), also known as B5R.2, is a 276 amino acid protein that contains one FAD-binding FR-type domain and belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Existing as two alternatively spliced isoforms, CYB5R2 reduces 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt and lucigenin in spermatozoa, which results in NADH-dependent lucigenin chemiluminescence. CYB5R2 binds FAD as a cofactor and is encoded by a gene located on human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.
Function:
NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1).
Tissue Specificity:
Restricted expression.
Similarity:
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Contains 1 FAD-binding FR-type domain.
Database links:Entrez Gene: 51700 Human
Entrez Gene: 320635 Mouse
Omim: 608342 Human
SwissProt: Q6BCY4 Human
SwissProt: Q3KNK3 Mouse
Unigene: 414362 Human
Unigene: 132828 Mouse
Unigene: 16198 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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