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Product Name Anti-Phospho-MAP3K8 (Ser400)/Gold Chinese Name 胶体金标记的磷酸化丝裂原活化蛋白激酶激酶8抗体 Alias MAP3K8 (phospho S400); P-MAP3K8/Tpl2 (Ser400); Phospho-MAP3K8(Ser400); Phospho-Tpl2 (Ser400); P-Tpl2 (Ser400);c COT; Cancer Osaka thyroid oncogene; Cancer Osaka thyroid oncogene; CCOT; COT; COT proto oncogene serine/threonine protein kinase; EST; ESTF; Ewing sarcoma transformant; FLJ10486; M3K8_HUMAN; MAP3K 8; MAP3K8; Mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Proto oncogene cCot; Proto-oncogene c-Cot; Serine/threonine protein kinase cot; Serine/threonine-protein kinase cot; TPL 2; TPL-2; TPL2; Tumor progression locus 2. Product Type Phosphorylated anti Research Area Tumour immunology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 53kDa Form Lyophilized or Liquid Concentration 0.4mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human MAP3K8/Tpl2 around the phosphorylation site of Ser400 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. Storage Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. Product Detail background:
This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
Function:
Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
Subunit:
Forms a ternary complex with NFKB1 and TNIP2.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Expressed in several normal tissues and human tumor-derived cell lines.
Post-translational modifications:
Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Database links:Entrez Gene: 1326 Human
Entrez Gene: 26410 Mouse
Omim: 191195 Human
SwissProt: P41279 Human
SwissProt: Q07174 Mouse
Unigene: 432453 Human
Unigene: 3275 Mouse
Unigene: 9939 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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