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Product Name Anti-phospho-Cytokeratin 8 (Ser23)/Gold Chinese Name 胶体金标记的磷酸化细胞角蛋白8抗体 Alias Cytokeratin 8 (phospho S23); p-Cytokeratin 8 (phospho S23); card2; Cardiac autoantigen 2 120kD; CK 8; CK-8; ck8; Cyk 8; cyk8; CYKER; Cytokeratin endo A; Cytokeratin-8; Cytokeratin8; DreK8; EndoA; k0; CYK8; k2c8; K2C8_HUMAN; k8; Keratin 8; Keratin type ii cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; Keratin8; KO; Krt 2.8; Krt 8; krt8; KRT-8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, ) Applications IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 53kDa Form Lyophilized or Liquid Concentration 0.4mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human CK8 around the phosphorylation site of Ser23 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. Storage Store at 2-8 ºC for 3-6 months. Avoid repeated freeze/thaw cycles. Product Detail background:
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].
Function:
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Subunit:
Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1.
Subcellular Location:
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
Tissue Specificity:
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Post-translational modifications:
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
DISEASE:
Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600].
Similarity:
Belongs to the intermediate filament family.
Database links:Entrez Gene: 3856 Human
Entrez Gene: 16691 Mouse
Omim: 148060 Human
SwissProt: P05787 Human
SwissProt: P11679 Mouse
Unigene: 533782 Human
Unigene: 708445 Human
Unigene: 358618 Mouse
Unigene: 11083 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
细胞角蛋白是一类与结构相关的蛋白家族,其在epithelial cells中形成Cytoskeleton中间丝。CK8存在于某些正常腺上皮及其Tumour,包括许多导管上皮和腺上皮,如结肠、胃、小肠、气管的上皮和尿路上皮。CK8主要用于腺癌和导管癌的诊断,该抗体特异识别第23位丝氨酸磷酸化的CK8。
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