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Product Name Anti-phospho-IRE1a (Ser 726)/FITC Chinese Name FITC标记的磷酸化内质网核Signal transduction蛋白a1抗体 Alias p-IRE1a (phospho-Ser 726); IRE1 (phospho S726) ; IRE1 alpha (p-Ser726); Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring protein 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; Protein kinase/endoribonuclease; Serine/threonine protein kinase/endoribonuclease IRE1.ERN1_HUMAN Product Type Phosphorylated anti Research Area Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, ) Applications Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 107kDa Cellular localization The cell membrane Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human IRE1a around the phosphorylation site of Ser 726 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Function:
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Subunit:
Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. Interacts with TAOK3 and TRAF2.
Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Tissue Specificity:
Ubiquitously expressed. High levels observed in pancreatic tissue.
Post-translational modifications:
Autophosphorylated.
Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
Database links:Entrez Gene: 2081 Human
Entrez Gene: 78943 Mouse
Omim: 604033 Human
SwissProt: O75460 Human
SwissProt: Q9EQY0 Mouse
Unigene: 133982 Human
Unigene: 592041 Human
Unigene: 700027 Human
Unigene: 20452 Mouse
Unigene: 340943 Mouse
Unigene: 226435 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Username | Quantity | bought time |
Ma*** | 2 | 2024-07-15 |
Ge*** | 1 | 2024-03-24 |
Xa*** | 1 | 2024-02-20 |
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