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Product Name Anti-phospho-Dynamin 1 (Ser778)/Cy7 Chinese Name Cy7标记的磷酸化酶动力蛋白1抗体 Alias Dynamin 1 (phospho S778); Dynamin 1 (phospho Ser778); p-Dynamin 1 (S778); p-Dynamin 1 (Ser778); B dynamin; D100; DNM 1; DNM; DNM1; DYN1_HUMAN; Dynamin; Dynamin-1; Dynamin1. Product Type Phosphorylated anti Research Area Cell biology Neurobiology Signal transduction Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Sheep, ) Applications
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 97kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human Dynamin 1 around the phosphorylation site of Ser778 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
Dynamin I is a GTPase enzyme required for the retrieval of synaptic vesicles after exocytosis and functions in endocytosis by stimulating assembly of invaginating synaptic vesicles (1). Dynamin I is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C (PKC) (2–5). The phosphorylation site in PKC-phosphorylated Dynamin I is a single site at Serine 795, which is located near a binding site for the SH3 domain of p85, the regulatory subunit of phosphatidylinositol 3-kinase (2–5). Dephosphorylation is required for synaptic vesicle retrieval, suggesting that phosphorylation affects the subcellular localization of Dynamin I (5). Mouse, rat and human Dynamin I are phosphorylated on serine residues, including Ser 778, by Cdk5, regulating PACSIN1 recruitment and enabling synaptic vesicle endocytosis.
Function:
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.
Subunit:
Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3 (By similarity). Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with SNX9. Interacts with MYO1E (via SH3 domain).
Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.
Similarity:
Belongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain.
Database links:
UniProtKB/Swiss-Prot: Q05193.2
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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