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Product Name Anti-MKP1/Cy7 Chinese Name Cy7标记的丝裂原活化蛋白激酶磷酸酶-1抗体 Alias DUSP1; Mitogen activated protein kinase phosphatase 1; CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP 1; DUSP-1; EC 3.1.3.16; EC 3.1.3.48; HVH 1; HVH1; MAP kinase phosphatase 1; MKP 1; MKP1; MKP-1; Protein tyrosine phosphatase CL100; PTPN 10; PTPN10; Serine/threonine specific protein phosphatase; VH 1; VH1; 3ch134; Mkp1; Ptpn16. Research Area Tumour immunology Signal transduction Apoptosis transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Dog, Cow, Rabbit, ) Applications
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 39kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DUSP1 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues.
Function:
Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
Tissue Specificity:
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
Post-translational modifications:
Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.
Similarity:
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain.
Database links:Entrez Gene: 1843 Human
Entrez Gene: 19252 Mouse
Omim: 600714 Human
SwissProt: P28562 Human
SwissProt: P28563 Mouse
Unigene: 171695 Human
Unigene: 239041 Mouse
Unigene: 98260 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
MKPs是一类丝氨酸/苏氨酸和酪氨酸双重底物特异性的磷酸酶 ,对于丝裂素活化蛋白激酶活性的调节起着十分重要的作用,可使丝裂素活化蛋白激酶上的苏氨酸/酪氨酸去磷酸化失活。目前研究发现MKPs分别有MKP-1、MKP-2、MKP-3及MKP4-6。
MKPs受MAPK信号通路中多种成分的诱导 ,决定了它与MAPK之间作用的特异性。 通过去磷酸化作用调节MAPK信号途径的活性 ,确保了细胞内信号的精确传递 ,参与了多种主要的细胞功能的调节。
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