TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Anti-PRDM1/ Blimp1/AF594 Chinese Name AF594标记的Blymphocyte诱导成熟蛋白1抗体 Alias Blimp 1; Blimp-1; B Lymphocyte Induced Maturation Protein 1; Beta interferon gene positive regulatory domain I binding factor; Beta-interferon gene positive regulatory domain I-binding factor; BLIMP-1; BLIMP1; Positive Regulatory Domain I Binding Factor 1; Positive regulatory domain I-binding factor 1; PR Domain Containing 1; PR domain containing 1 with ZNF domain; PR domain containing 1 with ZNF domain isoform 2; PR domain containing protein 1; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI binding factor 1; PRDI-BF1; PRDI-binding factor 1; PRDM 1; Prdm1; PRDM1_HUMAN. Research Area Cell biology immunology Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Chicken, Pig, Cow, Horse, Rabbit, ) Applications IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 92kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PRDM1/Blimp1 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated.
Function:
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells.
Subunit:
Interacts with PRMT5.
Subcellular Location:
Nucleus. Cytoplasm.
Similarity:
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain.
Database links:
Entrez Gene: 639 Human
Entrez Gene: 12142 Mouse
Omim: 603423 Human
SwissProt: O75626 Human
SwissProt: Q60636 Mouse
Unigene: 436023 Human
Unigene: 4800 Mouse
Unigene: 8012 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Scan Wechat Qrcode