TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Anti-VPS24/AF594 Chinese Name AF594标记的液泡蛋白分选蛋白24抗体 Alias VPS 24; VPS24; CGI 149; CGI149 protein; Charged multivesicular body protein 3; CHMP3; CHMP3_HUMAN; Chromatin modifying protein 3; Chromatin-modifying protein 3; HGNC:29865; hVps24; NEDF; Neuroendocrine differentiation factor; Vacuolar protein sorting 24 (yeast); Vacuolar protein sorting 24 homolog (S. cerevisiae); Vacuolar protein sorting associated protein 24; Vacuolar protein sorting-associated protein 24; VPS 24; VPS24. Research Area Cell biology Signal transduction Cell differentiation lymphocyte Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 25kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human VPS24 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. [provided by RefSeq, Nov 2010]
Function:
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Required for sorting/trafficking of EGF receptor.
Subcellular Location:
Cytoplasm > cytosol. Membrane. Endosome. Late endosome membrane. Localizes to the midbody of dividing cells.
Tissue Specificity:
Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Similarity:
Belongs to the SNF7 family.
Database links:Entrez Gene: 422918 Chicken
Entrez Gene: 51652 Human
Entrez Gene: 66700 Mouse
Entrez Gene: 406621 Zebrafish
Omim: 610052 Human
SwissProt: Q9Y3E7 Human
SwissProt: Q9CQ10 Mouse
SwissProt: Q6NY88 Zebrafish
Unigene: 591582 Human
Unigene: 181278 Mouse
Unigene: 162751 Rat
Unigene: 48763 Zebrafish
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Scan Wechat Qrcode