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Product Name Anti-FBLIM1/AF488 Chinese Name AF488标记的FBLIM1蛋白抗体 Alias 2410043F08Rik; Cal; CSX associated LIM; DKFZp434G171; FBLI1_HUMAN; FBLIM 1; Fblim1; FBLP 1; FBLP-1; FBLP1; Filamin binding LIM protein 1; Filamin-binding LIM protein 1; Gt10; MGC105665; MIG2 interacting protein;; MIG2-interacting protein; Migfilin; Mitogen inducible 2 interacting protein; Mitogen-inducible 2-interacting protein; OTTHUMP00000003118; OTTHUMP00000003119; OTTHUMP00000003120; OTTMUSP00000011062; OTTMUSP00000011063; RP11-169K16.5; RP23-291H3.1. Research Area Cell biology immunology Signal transduction The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Cow, Sheep, ) Applications ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 41kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FBLIM1 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function:
Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly.
Subcellular Location:
Cytoplasm > cell cortex. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. Associated with actin stress fiber at cell-ECM focal adhesion sites. Isoform 1 and isoform 3 are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. Isoform 2 is localized at actin stress fibers.
Tissue Specificity:
Isoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets.
Similarity:
Contains 3 LIM zinc-binding domains.
Database links:Entrez Gene: 54751 Human
Entrez Gene: 74202 Mouse
Omim: 607747 Human
SwissProt: Q8WUP2 Human
SwissProt: Q71FD7 Mouse
Unigene: 530101 Human
Unigene: 286536 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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