Product Name	Anti-NCKAP1/AF488
Chinese Name	AF488标记的膜相关的蛋白质HEM2抗体
Alias	NCK associated protein 1; NCK associated protein 1; Membrane associated protein HEM2; Membrane-associated protein HEM-2; NAP 1; NAP1; NAP125; NCK associated protein; Nck-associated protein 1; Nckap1; NCKP1_HUMAN; p125Nap1.
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction  Stem cells  Apoptosis  transcriptional regulatory factor  The cell membrane蛋白
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	(predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Guinea Pig, G)
Applications	IF=1:50-200  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Molecular weight	129kDa
Form	Lyophilized or Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human 12 NCKAP1
Lsotype	IgG
Purification	affinity purified by Protein A
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Product Detail	background: NAP125, also known as NCKAP1 (NCK-associated protein 1), p125Nap1 or membrane-associated protein HEM-2, is a 1,128 amino acid single pass membrane protein that exists as two alternatively spliced isoforms and belongs to the HEM-1/HEM-2 family. While widely expressed, NAP125 is found at highest levels in heart, brain and skeletal muscle where it regulates Rac-dependent actin remodeling as part of a lamellipodial complex with WAVE2, Abi-1 and CYFIP1. NAP125 localizes to the cytoplasmic side of lamellipodium membrane and is encoded by a gene that maps to human chromosome 2q32.1 and mouse chromosome 2 C3. NAP125 expression is markedly reduced in Alzheimer disease (AD)-affected brains, suggesting a possible role in the disease. Function: Is part of lamellipodial complex that controls Rac-dependent actin remodeling.Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Subcellular Location: Cell membrane. Cell projection Tissue Specificity: Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle. Similarity: Belongs to the HEM-1/HEM-2 family. Database links: Entrez Gene: 3071 Human Entrez Gene: 105855 Mouse Entrez Gene: 315348 Rat Omim: 141180 Human SwissProt: P55160 Human Unigene: 182014 Human Unigene: 30805 Mouse Unigene: 149002 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Product Picture	Blank control: HL60. Primary Antibody (green line): Rabbit Anti-GPR65 antibody (SL6204R-AF488) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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