Product Name	Anti-Phospho-Wee1(Ser123)/AF350
Chinese Name	AF350标记的磷酸化WEE1蛋白抗体
Alias	DKFZp686I18166; EC 2.7.10.2; FLJ16446; Wee 1; Wee1 homolog; WEE1 homolog S. pombe; Wee1 like protein kinase; Wee1 tyrosine kinase; Wee1+ homolog; Wee1+ S. pombe homolog; WEE1A; Wee1A kinase; WEE1hu; WEE1 tyrosine kinase isoform 1; MGC105683; WEE1_HUMAN.
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  Stem cells  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	(predicted: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, )
Applications	IF=1:50-200  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Molecular weight	72kDa
Form	Lyophilized or Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human Wee1 around the phosphorylation site of Ser123 [SS(p-S)PV]
Lsotype	IgG
Purification	affinity purified by Protein A
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Product Detail	background: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. Function: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase. Ubiquitinated and degraded at the onset of G2/M phase. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain. Database links: Entrez Gene: 7465 Human Entrez Gene: 22390 Mouse Entrez Gene: 308937 Rat Omim: 193525 Human SwissProt: P30291 Human SwissProt: P47810 Mouse SwissProt: Q63802 Rat Unigene: 249441 Human Unigene: 287173 Mouse Unigene: 208255 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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