Drug Names

Generic Name: Human 4-Aminobutyrate Aminotransferase (ABAT) ELISA Kit

Purpose

Our Human 4-Aminobutyrate Aminotransferase (ABAT) ELISA kit is to assay ABAT levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ABAT. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for ABAT is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ABAT and HRP conjugated ABAT antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ABAT. You can calculate the concentration of ABAT in the samples by comparing the OD of the samples to the standard curve.

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