Cat NO.:	EL0177Hu
Product:	Sunlong Medical™ Human Apolipoprotein H/Apo H ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	88% - 116%
Mean Spike Recovery:	1.03
CV of Intra plate:	2.4% - 6.3%
CV of Inter plate:	3.9% - 4.6%
NCBI_Gene:	350
UniProtKB:	P02749

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein H/Apo H monoclonal antibody Human Apolipoprotein H/Apo H freeze-dried standard Apolipoprotein H/Apo H detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo H antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate for incubation. After washing to remove unbound substances, the detection antibody labeled with horseradish peroxidase is added for further incubation. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo H in the samples.A stop solution is added to terminate the reaction, and the absorbance value ismeasured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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