Cat NO.:	EL0149Hu
Product:	Sunlong Medical™ Human Apolipoprotein D/Apo D ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	85% - 103%
Mean Spike Recovery:	0.91
CV of Intra plate:	2.0% - 8.1%
CV of Inter plate:	1.9%-2.8%
NCBI_Gene:	347
UniProtKB:	P05090

 

SAMPLE VOLUME	90 μL 1×Assay Buffer and 10 μL prediluted sample
SENSITIVITY	25.67 pg/mL
RANGE	0.63 ng/mL – 40 ng/mL
ASSAY TIME	3.5 h
RECOVERY	85% – 103%
AVERAGE RECOVERY	0.91
INTRA PRECISION	2.0% – 8.1%
INTER-PRECISION	1.9%-2.8%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein D/Apo D monoclonal antibody Human Apolipoprotein D/Apo D freeze-dried standard Apolipoprotein D/Apo D detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo D antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo D present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo D in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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