SAMPLE TYPE	Serum, plasma, cell culture supernatant, and other biological samples
SAMPLE VOLUME	5 μL
SENSITIVITY	0.51 pg/mL
RANGE	15.63 pg/mL – 1000 pg/mL
ASSAY TIME	3.5 h
RECOVERY	93% – 109%
AVERAGE RECOVERY	1.02
INTRA PRECISION	2.3% – 3.0%
INTER-PRECISION	1.7% – 2.2%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with specific anti-human BID antibody Human BID freeze-dried standard Streptavidin-HRP labeled human BID detect Antibody Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film Standard Diluent
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human BID antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, BID present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of BID in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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