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Sunlong Medical™ Mouse IL-6 ELISA Kit
Sunlong Medical™ Mouse IL-6 ELISA Kit
IL6 elisa kit | interleukin 6 elisa kit | Il-6
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Regular members: $455.2
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Cat NO.:
EL0107Mo
Product:
Sunlong Medical™ Mouse IL-6 ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
89% - 112%
Mean Spike Recovery:
1.02
CV of Intra plate:
2.1 % - 5.3 %
CV of Inter plate:
2.3 % - 3.0 %
NCBI_Gene:
16193
UniProtKB:
P08505
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Mouse IL-6 freeze-dried standard
IL-6 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-6 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-6 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 











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Ge***12024-09-10
Fi***22024-08-23
Be***12024-08-17
Ya***32024-08-14
Wi***22024-08-09
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