TEL: +86 571 56623320 EMAIL: [email protected]
Cat NO.:EL0091Mo Product:Sunlong Medical™ Mouse myeloperoxidase/MPO ELISA Kit Storage4℃ (unopened) for two years Shipping Condition4℃ Sample Type:Serum | plasma | cell culture supernatant and other biological samples Spike Recovery Range:91% - 107% Mean Spike Recovery:0.99 CV of Intra plate:3.8 % - 5.4 % CV of Inter plate:3.0 % - 4.1 % NCBI_Gene:17523 UniProtKB:P11247
PLATFORM ELISA PLATE Detachable 96-well plate SIZE 96T/48T STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. DELIVERY 4℃ blue ice transportation COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-MPO monoclonal antibody
Mouse MPO freeze-dried standard
MPO detect Antibody
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing FilmASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse MPO antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, MPO present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of MPO in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
Username | Quantity | bought time |
Is*** | 2 | 2024-08-17 |
Et*** | 1 | 2024-07-08 |
Scan Wechat Qrcode
Scan Whatsapp Qrcode