Cat NO.:	EL0017Hu
Product:	Sunlong Medical™ Human MMP-13 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:	81% - 112%
Mean Spike Recovery:	1
CV of Intra plate:	1.0 % - 1.9 %
CV of Inter plate:	4.5 % - 8.3 %
NCBI_Gene:	4322
UniProtKB:	P45452

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CD28 monoclonal antibody Human CD28 freeze-dried standard Human CD28 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CD28 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, CD28 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CD28 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

1. IL-37 alleviates intervertebral disc degeneration via the IL-1R8/NF-κB pathway

OSTEOARTHRITIS AND CARTILAGE 2023-01-21

 

2. Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes

BIOCHEMICAL PHARMACOLOGY 2023-12-21

 

3. Bone Mesenchymal Stem Cell-derived Exosomes Involved Co-delivery and Synergism Effect with Icariin via Mussel-inspired Multifunctional Hydrogel for Cartilage Protection

Asian Journal of Pharmaceutical Sciences 2023-03-31

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