Cat NO.:	EL0119Mo
Product:	Sunlong Medical™ Mouse GDF-15 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Spike Recovery Range:	81.7 %-106.1 %
Mean Spike Recovery:	0.92
CV of Intra plate:	7.1 % - 8.9 %
CV of Inter plate:	3.9 % - 8.1 %
NCBI_Gene:	Inquiry
UniProtKB:	Inquiry

 

PLATFORM	ELISA
PLATE	Detachable 96-well plate
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
ASSAY PRINCIPLE	This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-mouse GDF-15 antibodies are precoated on a high-affinity ELISA plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the plate and incubated, allowing the GDF-15 present in the samples to bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, HRP-labeled streptavidin is added. After washing again, a chromogenic substrate TMB is added for color development under light protection. The intensity of the color reaction is directly proportional to the concentration of GDF-15 in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

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