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Sunlong Medical™ Human IL-17A High Sensitivity ELISA Kit
Sunlong Medical™ Human IL-17A High Sensitivity ELISA Kit
IL17A elisa kit | interleukin 17A elisa kit | CTLA-8 | CTLA8 | cytotoxic T-lymphocyte-associated antigen 8 | cytotoxic T-lymphocyte-associated protein 8 | cytotoxic T-lymphocyte-associated serine esterase 8 | IL-17 | IL-17A | IL17 | ILA17 | interleukin 17
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  • NO.:HS-EL0190Hu
    Species:Human
    Assay range:0.78pg/mL-50pg/mL
    Sensitivity:0.20 pg/mL
    Sample Volume:Serum | plasma;20 μL;Cell culture supernatant;100 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:198
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Cat NO.:
HS-EL0190Hu
Product:
Sunlong Medical™ Human IL-17A High Sensitivity ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
80% - 95%
Mean Spike Recovery:
0.86
CV of Intra plate:
4.3% - 4.6%
CV of Inter plate:
5.9% - 9.0%
NCBI_Gene:
3605
UniProtKB:
Q16552

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-17A monoclonal antibody
Human IL-17A freeze-dried standard
IL-17A detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-17A antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-17A present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-17A in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).







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