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Sunlong Medical™ Human IL-21 High Sensitivity ELISA Kit
Sunlong Medical™ Human IL-21 High Sensitivity ELISA Kit
IL21 elisa kit | interleukin 21 elisa kit | CVID11 | IL-21 | interleukin-21 | interleukin-21 isoform | Za11
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  • NO.:HS-EL0152Hu
    Species:Human
    Assay range:9.38pg/mL-600pg/mL
    Sensitivity:1.03 pg/mL
    Sample Volume:Serum | plasma;20 μL;Cell culture supernatant;100 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:292
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Cat NO.:
HS-EL0152Hu
Product:
Sunlong Medical™ Human IL-21 High Sensitivity ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
90% - 116%
Mean Spike Recovery:
1.07
CV of Intra plate:
4.1% - 5.0%
CV of Inter plate:
2.6% - 4.2%
NCBI_Gene:
59067
UniProtKB:
Q9HBE4

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-21 monoclonal antibody
Human IL-21 freeze-dried standard
IL-21 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-21 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-21 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-21 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

 

JOURNAL OF ETHNOPHARMACOLOGY 2023-04-21
 
 
 
CHROMOSOMA 2023-05-25

 

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