Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

Kit features:

1, specifically detects Chlamydia pneumoniae and has no cross-reaction with other biological genomes.

2, high sensitivity, the lowest detection limit is about 0.13 IFU/response.

3. DNA polymerase adopts hot start method. It can inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, can be used Check the effectiveness of the kit.

5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.

Chlamydia pneumoniae belongs to the family Chlamydiaceaeem>), an obligate intracellular parasitic prokaryote, was first isolated in 1965 and officially named in 1989. Chlamydia pneumoniae infects the respiratory tract, including the throat, trachea and lungs, causing about 5-10% of community-acquired pneumonia; 70% of people infected have no or only mild symptoms, and 30% will have more severe respiratory symptoms, including Community-acquired pneumonia, bronchitis, and upper respiratory tract infections. The infection easily becomes chronic and is related to many chronic diseases, such as chronic obstructive pulmonary disease, bronchial asthma, lung cancer, neurological disorders, multiple sclerosis, schizophrenia, coronary heart disease, atherosclerosis, sarcoidosis and reactions. arthritis, etc. After Chlamydia pneumoniae invades the human body, it mainly causes a monocyte-macrophage reaction. Alveolar macrophages serve as carriers for the storage and spread of the pathogen, causing its persistent infection in the host body. In animal experimental studies of non-human mammals such as mice and monkeys, it has been found that the infection is often asymptomatic in the early stage, and most lung lesions appear within 2 months, mainly manifesting as interstitial pneumonia, with local multinucleated cell infiltration in the early stage, and later It is infiltration of macrophages and lymphocytes. Its pathogenesis remains unclear. Chlamydia pneumoniae can be isolated from the lungs and spleen. Chlamydia pneumoniae is transmitted through respiratory droplets, and asymptomatic infections can also be transmitted. There are no reports of transmission between animals and humans. It is generally believed that humans are the only host of Chlamydia pneumoniae; however, in koalas, horses, and other marsupials Chlamydia pneumoniae has also been found in animals, amphibians, and reptiles. According to genetic analysis, human Chlamydia pneumoniae originated from animals, but it no longer relies on animals for transmission.

Culture and serological methods can be used to detect Chlamydia pneumoniae. Chlamydia pneumoniae can only be cultured in tissue, not in cell-free culture systems. It is not sensitive to chicken embryos. It is generally grown in cells derived from the respiratory tract, and is more difficult to culture than other chlamydiae. The most commonly used serological method is the complement fixation reaction. Generally, the heat-treated chlamydia suspension is used as the antigen to determine the serum being tested . Complement-fixing antibodies generally appear in mammals 7 days after infection. Microimmunofluorescence antibody detection technology is specific and highly sensitive for the diagnosis of Chlamydia pneumoniae. Therefore, it is praised by domestic and foreign scholars as the "gold standard" for the diagnosis of Cpn infection. However, the antigen sheet production process of this method is complicated, and the laboratory The requirements are high. PCR is a method of enzymatically synthesizing specific DNA fragments in vitro. It is more sensitive than other methods and has strong specificity. It can determine the type of bacterial infection. The detection speed is fast and can be completed in only two or three hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

This kit designs primers and probes based on the sequence of extracellular membrane protein genes. It can specifically identify Chlamydia pneumoniae. It has been verified by BLAST that there is no cross-reaction with the genomes of other organisms. This kit detected 14 types of bacteria and 19 types of viruses, and no non-specific signals were found. 186 respiratory clinical samples were tested and 51 positive results were obtained. It can be seen that this kit can be used for the detection and identification of Chlamydia pneumoniae.

This product is for research use only.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测肺炎衣原体，与其他生物基因组无交叉反应。

2， 灵敏度高，最低检测极限约为0.13 IFU/反应。

3， DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品，可用于检验试剂盒有效性。

5， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

肺炎衣原体（Chlamydia pneumoniae）是属于衣原体科（Chlamydiaceae）的专性细胞内寄生原核生物，在1965年首次分离出来，1989年被正式命名。肺炎衣原体感染呼吸道，包括咽喉、气管和肺，引起约5-10%的社区获得性肺炎；感染后70%的人没有或只有轻度症状，30%的人会有较严重的呼吸道症状，包括社区获得性肺炎、支气管炎和上呼吸道感染。其感染易转为慢性，与许多慢性疾病有关，如慢性阻塞性肺病、支气管哮喘、肺癌、神经功能紊乱、多发性硬化症、精神分裂症、冠心病、动脉粥样硬化、结节病及反应性关节炎等。肺炎衣原体侵入人体后，主要引起单核-巨噬细胞反应，肺泡巨噬细胞作为病原体贮存和传播的载体，造成其在宿主体内的持续感染。在非人哺乳动物如小鼠及猴的动物实验研究中发现，感染早期多无症状，大部分在2个月出现肺部病变，主要表现为间质性肺炎，早期局部有多核细胞浸润，以后则为巨噬细胞和淋巴细胞浸润。其发病机制尚不明确。可从肺部及脾脏中分离出肺炎衣原体。肺炎衣原体通过呼吸道飞沫进行传播，无症状感染者也可进行传播，没有动物和人之间传播的报道，一般认为人类是肺炎衣原体唯一的宿主；但在考拉、马，以及其他有袋类动物、两栖动物、爬行动物中也有发现肺炎衣原体。根据遗传学分析，人类的肺炎衣原体是源于动物，但已不依赖于动物进行传播。

 对肺炎衣原体的检测可以采用培养法和血清学方法。肺炎衣原体只能使用组织培养，不能使用无细胞培养体系，对鸡胚不敏感，一般是在呼吸道来源的细胞内进行，培养较其他衣原体更为困难。最常用的血清学方法是补体结合反应。一般用加热处理过的衣原体悬浮液作为抗原（属特异抗原）来测定被检血清（属特异血清）。哺乳动物一般于感染后7天出现补体结合抗体。微量免疫荧光抗体检测（MIF）技术对肺炎衣原体的诊断是特异的且敏感性高，因此被国内外学者誉为Cpn感染诊断的“金标准”，但该方法的抗原片制作过程复杂，实验室要求条件高。PCR是一种体外酶促合成特异性DNA 片段的方法,灵敏度高于其他方法，特异性强，可以确定感染的细菌种类，检测速度快，只需两三个小时即可完成。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒针对胞膜外蛋白基因的序列设计引物和探针，可以特异性识别肺炎衣原体，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了14种细菌和19种病毒，未发现非特异性信号；对186个呼吸道临床样本进行检测，获得51个阳性。可见本试剂盒可用于肺炎衣原体的检测和鉴定。

本产品仅供研究使用。

 

 

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