Details
Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.
Kit features:
1, Specific detection of Waddlia chondrophila, no cross-reaction with other biological genomes.
2, high sensitivity, the lowest detection limit is about 8 genomes/reaction.
3. DNA polymerase adopts hot start method. It can inhibit non-specific amplification and reduce background fluorescence.
4, with positive control sample, can be used Check the effectiveness of the kit.
5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.
Chlamydia pecorum, also known as chlamydiaphilia or chlamydia pecorum, also known as chlamydia ruminant,Chlamydia bovis, Chlamydia bovis, is a species belonging to the family Chlamydiaceae prokaryotes, obligate intracellular parasites, originally classified as Chlamydia psittaci serotype 2, was officially established as a new species in 1992; the hosts are mainly ruminants, such as cattle, sheep and goats etc., can also infect koalas and pigs. Infection can lead to serious illness, including: polyarthritis, enteritis, keratoconjunctivitis, pneumonia, endometritis, and occasionally encephalomyelitis. Fever, fatigue, purulent vaginal discharge, mild symptoms have also been reported. Symptoms such as vaginitis. Artificial infection can also cause metritis and salpingitis. Veterinary chlamydia is associated with urinary tract infections, infertility, and miscarriage. The pathogenic mechanism of veterinary chlamydia is unclear, but its presence is often detected in the digestive tract and reproductive tract of healthy ruminants.
Detection of animal chlamydia Generally, PCR method is used. PCR is a method of enzymatically synthesizing specific DNA fragments in vitro. It is more sensitive than other methods and has strong specificity. It can determine the type of bacterial infection. The detection speed is fast and can be completed in only two or three hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.
This kit targets the extracellular membrane of veterinary chlamydia Primers and probes were designed based on the gene sequence of the protein, which can specifically identify animal chlamydia. It has been verified by BLAST that there is no cross-reaction with the genomes of other organisms. This kit detected 6 types of other chlamydia and 14 types of bacteria, and no non-specific signals were found. 351 chlamydia-positive DNA samples from 9 kinds of animals were tested, and 24 animal chlamydia-positive ones were obtained, respectively from pigs and cattle. , goats and sheep. It can be seen that this kit is suitable for the detection and identification of chlamydia in animals.
-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。
试剂盒特点：
1， 特异性检测兽类衣原体，与其他生物基因组无交叉反应。
2， 灵敏度高，最低检测极限约为8个基因组/反应。
3， DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。
4， 带有阳性对照样品，可用于检验试剂盒有效性。
5， 带有UDG酶和dUTP，可降低残留DNA的污染。
兽类衣原体（Chlamydia pecorum），或称兽类嗜衣原体、兽类亲衣原体（Chlamydophila pecorum），也有称为反刍动物衣原体、牛羊亲衣原体、牛羊衣原体，是一种属于衣原体科（Chlamydiaceae）的原核生物，专性细胞内寄生，原先被归类为鹦鹉热衣原体血清型2（Chlamydia psittaci，serotype 2），于1992年正式确立为新物种；宿主主要是反刍动物，如：牛，绵羊和山羊等，也可以感染树袋熊和猪。其感染可以导致严重的疾病，包括：多发性关节炎、肠炎、角膜结膜炎、肺炎、子宫内膜炎，以及偶尔出现的脑脊髓炎，也有报道出现发烧、乏力、脓性阴道分泌物、轻度阴道炎等症状。人工感染时还可引起子宫炎和输卵管炎。兽类衣原体与尿路感染、不育和流产有关。兽类衣原体的致病机制尚不清楚，在健康反刍动物的消化道和生殖道也经常检测到其存在。
对兽类衣原体的检测一般采用PCR法。PCR是一种体外酶促合成特异性DNA 片段的方法,灵敏度高于其他方法，特异性强，可以确定感染的细菌种类，检测速度快，只需两三个小时即可完成。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。
本试剂盒针对兽类衣原体胞膜外蛋白的基因序列设计引物和探针，可以特异性识别兽类衣原体，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了6种其他衣原体和14种细菌，未发现非特异性信号；对源自9种动物的351个衣原体阳性DNA样品进行检测，获得24个兽类衣原体阳性，分别来自于猪、牛、山羊和绵羊。可见本试剂盒适用于兽类衣原体的检测和鉴定。
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