Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

Kit features:

1, Specific detection of Waddlia chondrophila, no cross-reaction with other biological genomes.

2, The sensitivity is higher than the ordinary PCR method, the lowest The detection limit is approximately 170 copies/reaction.

3. DNA polymerase adopts hot start method. It can inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, can be used Check the effectiveness of the kit.

5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.

There are four common types of chlamydia that infect pigs: Chlamydia suis, Chlamydia psittaci, Chlamydia abortus and Chlamydophila pecorum ), among which Chlamydia suis has the highest positive rate. Chlamydia suis was initially considered to be a variant of Chlamydia trachomatis in pigs, and was officially named an independent species in 1999. Pigs are generally believed to be its only host, but in recent years It is also found in cattle and frogs; its genetic material has also been found in human clinical samples, and there are no reports of causing disease. Because Chlamydia suis and Chlamydia trachomatis are closely related in genetic relationship, Therefore, the possibility of infecting humans cannot be ruled out. Chlamydia suis sometimes causes no symptoms after infecting domestic pigs or wild boars. Possible symptoms include: dyspnea, pneumonia, conjunctivitis, arthritis, pericarditis, polyserositis, enteritis, Diarrhea and reproductive dysfunction; experimental infection can cause conjunctivitis, respiratory and gastrointestinal damage in pigs. Through horizontal gene transfer, Chlamydia suis can acquire antibiotic resistance from other bacteria and relies on the resistance gene island It is the first acquired resistance factor identified.

 

Chlamydia suis It is difficult to culture in chicken embryos or in vitro cells; serological methods are commonly used for detection, such as complement fixation test, indirect hemagglutination test, enzyme-linked immunosorbent assay, etc. These methods are often more sensitive Low, poor specificity, and cannot detect the early stage of infection. PCR is a method of enzymatically synthesizing specific DNA fragments in vitro. It has higher sensitivity than other methods and strong specificity. It can determine the type of bacterial infection. The detection speed is fast and only It takes two to three hours to complete, which can make up for the shortcomings of the above methods. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

 

This kit designs primers and probes based on the extracellular membrane protein gene sequence to specifically identify Chlamydia suis. Verified by BLAST, there is no cross-reactivity with other biological genomes. Use thisThe kit detected 6 other types of chlamydia and found no non-specific signals; 225 pig tissue samples were tested and 42 positive results were obtained, which was higher than the 36 detected by the ordinary PCR method, showing higher sensitivity. It can be seen that this kit is suitable for the detection and identification of porcine chlamydia.

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1，   特异性检测猪衣原体，与其他生物基因组无交叉反应。

2，   灵敏度高于普通PCR法，最低检测极限约为170个拷贝/反应。

3，   DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4，   带有阳性对照样品，可用于检验试剂盒有效性。

5，   带有UDG酶和dUTP，可降低残留DNA的污染。

 

常见的感染猪的衣原体有四种：猪衣原体（Chlamydia suis）、鹦鹉热衣原体（Chlamydophila psittaci）、流产衣原体（Chlamydophila abortus）和兽类衣原体（Chlamydophila pecorum），其中阳性率最高的是猪衣原体。猪衣原体起初被认为是沙眼衣原体在猪当中的一个变种，于1999年被正式命名为独立物种。一般认为猪是其唯一宿主，但是近年在牛和青蛙中也有发现；在人类的临床样本中也发现其遗传物质，尚无致病报道。由于猪衣原体和沙眼衣原体（Chlamydia trachomatis）在亲缘关系上较近，因此不能排除感染人类的可能性。猪衣原体感染家猪或野猪后有时不引起症状，可能引起的症状包括：呼吸困难、肺炎、结膜炎、关节炎、心包炎、多浆膜炎、小肠炎、腹泻和生殖功能紊乱；实验性感染可引起猪的结膜炎、呼吸系统和胃肠道损伤。通过基因水平转移，猪衣原体可从其他细菌获得抗生素抗性，所依赖的抗性基因岛(TetR)是第一个被鉴别出来的获得性抗性因子。

猪衣原体在鸡胚或体外细胞中培养较为困难；血清学方法常用于检测，如：补体结合试验(CFT)、间接血凝试验(IHA)、酶联免疫吸附试验(ELISA)等，这些方法往往灵敏度较低，特异性较差，且不能检测感染早期。PCR是一种体外酶促合成特异性DNA 片段的方法,灵敏度高于其他方法，特异性强，可以确定感染的细菌种类，检测速度快，只需两三个小时即可完成，可以弥补上述方法的缺点。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒针对胞膜外蛋白基因序列设计引物和探针，特异性识别猪衣原体，经BLAST验证，与其他生物基因组没有交叉反应。使用本试剂盒检测了6种其他衣原体，未发现非特异性信号；对225个猪组织样本进行检测，获得42个阳性，高于普通PCR法检出的36个，显示出更高的灵敏度。可见本试剂盒适用于猪衣原体的检测和鉴定。

 

 

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