Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit features:

1, Specific detection of Waddlia chondrophila, no cross-reaction with other biological genomes.

2, high sensitivity, the lowest detection limit is about 80 genomes/reaction.

3. DNA polymerase adopts hot start method. It can inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, can be used Check the effectiveness of the kit.

5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.

 

Chlamydia felis, also known as Chlamydia felis/Chlamydia felis, is a specialized Sexual intracellular parasitic prokaryote, Gram-negative, belonging to the family Chlamydiaceae, once classified as a subtype of Chlamydophila psittaci in cats, was officially established as an independent species in 1999. It is generally believed that the host of Chlamydia felis is limited to cats, but it is also found in dogs and may also cause keratoconjunctivitis in humans; Chlamydia felis cannot survive after leaving the host, so it must be transmitted through close contact , young cats are more susceptible to infection. Feline chlamydia is a common infection and the main cause of feline conjunctivitis. The eye symptoms include conjunctivitis first on one side and then on both sides, nictitating membrane congestion, blepharospasm, increased eye secretions, and conjunctival edema. In addition, it can also cause Fever, lethargy, lameness, weight loss, and some upper respiratory symptoms. Close contact with cats can cause human illnesses such as conjunctivitis, although the probability is very low and caution is required when handling infected cats.

 

Chlamydia felis can be cultured in chicken embryos or cell lines. For the detection of feline chlamydia, the culture method is more difficult because the conjunctival swab contains only a small amount of bacteria, and the antibodies in the tears may also have an impact during chronic infection; and the culture method is time-consuming and complicated to operate. . The PCR method is an in vitro enzymatic synthesis method of specific DNA fragments. It is more suitable for detecting feline chlamydia. Its sensitivity is higher than the culture method, and it has strong specificity and fast detection speed, which can be completed in only two or three hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

 

This kit designs primers and probes for the extracellular membrane protein gene sequence. It can specifically identify Chlamydia felis and has been verified by BLAST to have no cross-reactivity with other biological genomes. This kit detected 5 other chlamydia and 14 types of bacteria, and no non-specific signals were found; 272 samples from 9 domestic animals were tested and 139 positive results were obtained, of which 135 were from cats and 4 were from affected conjunctiva. Inflamed dog. It can be seen that this kit is suitable for the detection and identification of feline chlamydia.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测猫衣原体，与其他生物基因组无交叉反应。

2， 灵敏度高，最低检测极限约为80个基因组/反应。

3， DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品，可用于检验试剂盒有效性。

5， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

猫衣原体（Chlamydia felis），或称猫嗜衣原体/猫亲衣原体（Chlamydophila felis），是一种专性细胞内寄生原核生物，革兰氏阴性，属于衣原体科（Chlamydiaceae），曾被归属为鹦鹉热嗜衣原体（Chlamydophila psittaci）在猫中的亚型，于1999年正式确立为独立物种。一般认为猫衣原体的宿主仅限于猫，但在犬中也有发现，在人类也可能引起角膜结膜炎；猫衣原体离开宿主后不能存活，因此必须通过近距离接触（通常是借助眼分泌物）才能传播，年轻猫（尤其是9个月龄以下）更易受到感染。猫衣原体属于常见感染，是猫结膜炎的主要病因，眼部症状表现为先单边后两边的结膜炎、瞬膜充血、眼睑痉挛、眼分泌物增多、结膜水肿，除此之外还可以引起发烧、昏睡、跛行、体重下降，以及一些上呼吸道症状。与猫的密切接触可能引起人类的疾病，如结膜炎，尽管概率非常低，处理受感染猫时仍需加以注意。

猫衣原体可以在鸡胚或细胞系中进行培养。对猫衣原体的检测，培养法较为困难，因为结膜拭子中只含有很少量的菌体，并且在慢性感染时眼泪中的抗体也会造成影响；而且培养法较为耗时，操作也比较复杂。PCR法是一种体外酶促合成特异性DNA 片段的方法,更适合检测猫衣原体，其灵敏度高于培养法，且特异性强，检测速度快，只需两三个小时即可完成。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒针对胞膜外蛋白基因序列设计引物和探针，可以特异性识别猫衣原体，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了5种其他衣原体和14种细菌，未发现非特异性信号；对源自9种家养动物的272个样本进行检测，获得139个阳性，其中135个来自猫，4个来自患结膜炎的狗。可见本试剂盒适用于猫衣原体的检测和鉴定。

 

 

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