Details
Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.
Kit features:
1, Specific detection of Waddlia chondrophila, no cross-reaction with other biological genomes.
2. Using hot-start Taq enzyme, you can Inhibit non-specific amplification and reduce background fluorescence.
3, with positive control sample, can be used Check the effectiveness of the kit.
4, with ROX reference dye, help Correct for loading errors and tube-to-tube differences.
5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.
Chlamydia trachomatis belongs to the family Chlamydiaceae is an obligate intracellular parasitic prokaryote, Gram-negative, first isolated in 1955 and officially named in 1973. Chlamydia trachomatis is widely distributed around the world, and its host is limited to humans. In most cases, there are no symptoms after infection. Without treatment, it can cause trachoma, genitourinary tract infections and lymphogranuloma venereum. Currently, there are 20 known serotypes, with different serotypes Cause different diseases. Serotypes A, B, Ba and C only infect the ocular mucosa and cause conjunctivitis . These serotypes are rarely isolated from the genitourinary tract; serotypes D, Da, E, F, G, H, I, Ia, J, Ja and K can infect the mucosal tissues of the genitourinary tract and eyes, causing urethritis, epididymitis, pelvic inflammatory disease, endometritis, vaginitis, ectopic pregnancy, fallopian tube infertility, proctitis, conjunctiva inflammatory disease, neonatal pneumonia, etc.; serotypes L1, L2, L2a, L2b and L3 are invasive and can infect lymph nodes through mucosal tissue and cause lymphogranuloma venereum. Chlamydia trachomatis is the leading cause of blindness and the most common sexually transmitted bacterium, infecting an estimated 500 million people worldwide every year. Chlamydia trachomatis can be transmitted sexually, through daily necessities, equipment, etc. It can also be transmitted by swimming. Mothers and infants can be transmitted through birth canal contact infection, intrauterine infection and puerperal infection.
Chlamydia trachomatis can be detected through in vitro culture, with good specificity but sensitivity Low, the test is more complex. Serological methods can use direct fluorescent antibody detection, enzyme-labeled immunoreaction, complement fixation test, ELISA, agglutination test, etc. PCR is an in vitro enzymatic method for synthesizing specific DNA fragments. Its sensitivity and specificity are better than other methods. The detection speed is fast and can be completed in only two to three hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution. The quantitative PCR method is divided into two indicating methods: dye and probe. On the premise of ensuring primer specificity, the dye method can achieve the same detection effect at a lower cost.
This kit uses dye method quantitative PCR to target the outer cell membrane protein gene sequence Design primers that can specifically identify all serotypes of Chlamydia trachomatis, as well as Chlamydia suis and Chlamydia muridarum ; verified by BLAST, and There is no cross-reactivity with other biological genomes. Since the hosts of these three types of chlamydia are humans, pigs and mice respectively, and all have host specificity, they are generally not detected during detection.cause interference. This kit was used to detect 11 types of bacteria with similar distribution ranges, and no non-specific signals were found. 17 human cervical swab samples were tested and 15 positive results were obtained. This kit is suitable for the detection of Chlamydia trachomatis.
-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。
试剂盒特点：
1， 特异性检测沙眼衣原体，也可检测猪衣原体和鼠衣原体。
2， 使用热启动的Taq酶，可抑制非特异性扩增，降低背景荧光。
3， 带有阳性对照样品，可用于检验试剂盒有效性。
4， 带有ROX参比染料，帮助校正加样误差和管间差异。
5， 带有UDG酶和dUTP，可降低残留DNA的污染。
沙眼衣原体（Chlamydia trachomatis）属于衣原体科（Chlamydiaceae），是一种专性细胞内寄生的原核生物，革兰氏阴性，于1955年首次分离，1973年正式命名。沙眼衣原体在全球范围内广泛分布，宿主限于人类，多数情况下感染后没有症状，不经治疗可引起沙眼、泌尿生殖道感染和性病淋巴肉芽肿，目前已知有20种血清型，不同血清型引起不同的疾病。血清型A、B、Ba和C只感染眼粘膜，引起结膜炎（沙眼），这些血清型很少能从泌尿生殖道分离得到；血清型D、Da、E、F、G、H、I、Ia、J、Ja和K可感染泌尿生殖道和眼部的粘膜组织，引起尿道炎、附睾炎、盆腔炎症、子宫内膜炎、阴道炎、异位妊娠、输卵管性不孕、直肠炎、结膜炎，以及新生儿肺炎等；血清型L1、L2、L2a、L2b和L3则具有侵入性，可穿过粘膜组织感染淋巴结，引起性病淋巴肉芽肿。沙眼衣原体是最主要的致盲性因素，也是最常见的性传播细菌，全球每年估计感染人数约为5亿人。沙眼衣原体可通过性传播，也可通过日常用品、器械等传播，游泳也可传播，母婴可以通过产道接触感染、宫内感染及产褥期感染进行传播。
沙眼衣原体可通过体外培养进行检测，特异性好，但敏感性低，试验较复杂。血清学方法可以采用直接荧光抗体检测、酶标免疫反应、补体结合试验、ELISA、凝集试验等。PCR是一种体外酶促合成特异性DNA 片段的方法，灵敏度和特异性均好于其他方法，检测速度快，只需两三个小时即可完成。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。定量PCR法又分为染料和探针两种指示方式，在保证引物特异性的前提下，染料法可以用更低的成本达到同样的检测效果。
本试剂盒采用染料法定量PCR，针对外胞膜蛋白基因序列设计引物，可以特异性识别沙眼衣原体所有血清型，同时也可识别猪衣原体（Chlamydia suis）和鼠衣原体（Chlamydia muridarum）；经BLAST验证，与其他生物基因组没有交叉反应。由于这三种衣原体的宿主分别为人、猪和鼠，且均有宿主特异性，检测时一般不会造成干扰。使用本试剂盒检测了11种分布范围类似的细菌，未发现非特异性信号；对17个人宫颈拭子样本进行检测，获得15个阳性。本试剂盒适用于沙眼衣原体的检测。
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