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Store in the dark at -20℃, transport at 2-8℃. Specific detection of Chlamydia/Chlamydiaphila, only related to a few species of bacteria have weak cross-reactivity and no cross-reactivity with other organism genomes outside the order Chlamydiales.
2. High sensitivity, the lowest detection limit is about Reacted to 56 genomes.
3. DNA polymerase adopts hot start method. It can inhibit non-specific amplification and reduce background fluorescence.
4, with positive control sample, can be used Check the effectiveness of the kit.
5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.
Chlamydiaceae belongs to the Chlamydiae phylum and the Chlamydiales order) and was first established in 1966. In 1999, based on the RNA gene sequences of only four members, they wereDivided into two species: Chlamydia and Chlamydophila . As more chlamydia species were discovered, the differences between the two species were no longer obvious, so in 2010 they were officially merged into one genus, still called Chlamydia . . Chlamydia was first discovered in 1935. Currently, about 15 species have been discovered, all of which are obligate intracellular parasites and can cause diseases in humans and animals. Chlamydia has been found in humans, mammals, birds and reptiles. Many chlamydiae can infect multiple hosts, while some are host-specific and infect only one type of host or type of host. Chlamydia that can infect humans include: Chlamydia trachomatis, Chlamydia pneumoniae, Chlamydia psittaci, Chlamydia abortus, etc. Chlamydia mainly invades the mucous membranes of the respiratory tract, gastrointestinal tract or reproductive system. The site and severity of infection are related to the type of chlamydia and the host. After infection, it will cause conjunctivitis and nasal mucositis in most animals, or it will invade the reproductive system and cause abortion and infertility, but sometimes it will not show symptoms. Birds infected with Chlamydia psittaci often have a subclinical state and may develop clinical symptoms under stress, manifesting as severe pneumonia and organ failure. Humans can become infected while raising birds, producing flu-like symptoms, often leading to misdiagnosis and serious consequences.
Chlamydia can be detected by culture and serological methods. Since chlamydia is an obligate intracellular parasite, it must be cultured in living tissues or cells. The operation is complex, time-consuming, has low sensitivity, and is difficult toto differentiate between chlamydial species. Serological methods have low sensitivity and are prone to cross-reactivity. The PCR method has the advantages of high sensitivity, strong specificity, and fast detection speed, which can be completed in only two to three hours, which can effectively make up for the shortcomings of the above two methods.
This kit designs specific primers and The probe can specifically identify various chlamydiae/Chlamydiaphila. It has been verified by BLAST that it is only compatible with a few species of Chlamydiales order bacteria have weak cross-reactivity and no cross-reactivity with other biological genomes. At high concentrations, these Chlamydiales bacteria may produce specific signals. This kit tested 145 biological samples, including conjunctival swabs, nasal swabs, milk, fresh tissues, body fluids, feces and other types, and obtained 125 positive results, which were basically consistent with the DNA microarray test results. 6 species of chlamydia, and the sequencing results confirmed that they included an unreported new species of chlamydia.
-20℃避光保存，2-8℃运输 特异性检测衣原体/嗜衣原体属，仅与衣原体目（Chlamydiales order ）细菌的少数几种（Estrella lausannensis，Waddlia chondrophila，Simkania negevensis）有微弱交叉反应，与衣原体目以外的其他生物基因组无交叉反应。
2， 灵敏度高，最低检测极限约每反应56个基因组。
3， DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。
4， 带有阳性对照样品，可用于检验试剂盒有效性。
5， 带有UDG酶和dUTP，可降低残留DNA的污染。
衣原体科（Chlamydiaceae）属于衣原体门（Chlamydiae phylum）衣原体目（Chlamydiales order），于1966年首次确立。1999年根据仅有的4个成员的RNA基因序列，将其分为两个种属：衣原体属（Chlamydia）和嗜衣原体属(Chlamydophila)。随着更多衣原体种类的发现，两个种属之间的区别不再明显，因此2010年正式将二者重新合并为一个种属，仍称为衣原体属（Chlamydia）。衣原体最早发现于1935年，现在已发现的有约15种，全部是专性细胞内寄生，在人和动物中可引起疾病。目前已在人、哺乳动物、鸟类和爬行类动物中发现衣原体。很多衣原体可感染多种宿主，有些则具有宿主特异性，只感染一种或一类宿主。可感染人类的衣原体有：沙眼衣原体（Chlamydia trachomatis），肺炎衣原体（Chlamydophila pneumoniae），鹦鹉热衣原体（Chlamydophila psittaci），流产衣原体（Chlamydophila abortus）等。衣原体主要侵袭呼吸道、胃肠道或生殖系统的粘膜，感染位点和严重程度与衣原体种类和宿主都有关系。感染后在多数动物中会引起结膜炎和鼻粘膜炎，或者侵袭生殖系统导致流产和不育，但有时也并不表现出症状。鸟类感染鹦鹉热衣原体后常处于亚临床状态，在压力下可能出现临床症状，表现为重度肺炎和器官衰竭。人类可在养鸟过程中被感染，产生类似流感的症状，常造成误诊导致严重后果。
衣原体可以通过培养法和血清学方法进行检测。由于衣原体是专性细胞内寄生，因此必须在活组织或细胞内进行培养，操作复杂，耗时，灵敏度低，并且难以区分衣原体种类。血清学方法的灵敏度较低，且容易产生交叉反应。PCR法具有灵敏度高、特异性强的优点，检测速度快，只需两三个小时即可完成，可以有效弥补上述两种方法的不足。
本试剂盒针对16S-23S rRNA基因区间序列设计特异性引物和探针，可以特异性识别各种衣原体/嗜衣原体，经BLAST验证，仅与衣原体目（Chlamydiales order ）细菌的少数几种（Estrella lausannensis，Waddlia chondrophila，Simkania negevensis）有微弱交叉反应，与其他生物基因组没有交叉反应。在高浓度的情况下，这几种衣原体目细菌有可能产生特异性信号。本试剂盒检测了145个生物样品，包括结膜拭子、鼻腔拭子、奶、新鲜组织、体液和粪便等各种类型，获得125个阳性，与DNA微点阵法检测结果基本一致，检出6种衣原体，配合测序结果证实其中包含一种未报道过的新衣原体物种。
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