Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

 Mycoplasma genitalium is a sexually transmitted pathogenic bacterium that lives on the ciliated epithelial cells of the human urinary and reproductive tracts. Mycoplasma genitalium was first isolated from the human urogenital tract in 1981 and was identified as a new mycoplasma species in 1983. It occurs more frequently than other common sexually transmitted infections, occurs more frequently in both men and women, and is a recognized risk factor for HIV transmission, affecting gay men and men who have previously received the antibiotic azithromycin. The rate is higher. It causes urethritis in both men and women, and cervicitis and pelvic inflammatory disease in women. Its complete genome sequence was published in 1995. It is 0.58 Mb in size and contains 475 genes. It is the second most common genome sequence after Haemophilus influenzae . A completely sequenced bacterial genome. Mycoplasma genitalium and Mycoplasma pneumoniae share common antigens, resulting in cross-reactivity in most serological experiments.

Mycoplasma genitalium has extremely high requirements on the culture medium and grows slowly, making it difficult to culture. In particular, culture from clinical specimens is less likely to be successful, which makes detection of pathogens in clinical specimens and subsequent isolation and culture very difficult. Since there are no specific serological tests, nucleic acid amplification testing is the only viable option for detecting M. genitalium. The PCR method has the advantages of high sensitivity and specificity, and the detection time is short, and the results can be obtained in just a few hours.

The Mycoplasma genitalium PCR detection kit selects a sequence of the adhesin protein gene After PCR identification, the primer was verified by BLAST to specifically target Mycoplasma genitalium, but at the same time it produced a cross-band of different sizes and easy to distinguish with Haemophilus influenzae, but had no cross-reactivity with the genomes of other organisms. This kit was used to detect 16 species of mycoplasma and 17 species of bacteria with close distribution range or close relationship. Only Mycoplasma genitalium produced a specific amplification band, and Haemophilus influenzae produced a smaller and easily distinguishable band.. It can be seen that this kit is species-specific and can be used for the identification and detection of Mycoplasma genitalium.

This kit is for scientific research only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

生殖支原体（Mycoplasma Genitalium）是一种性传播的致病菌，生活在人类尿道和生殖道的纤毛上皮细胞上。1981年首先从人类的泌尿生殖道中分离出生殖支原体，于1983年被确定为新的支原体物种。它的发病率高于其他常见的性传播感染，在男性和女性中都有较高的发病率，也是艾滋病毒传播的一个公认的危险因素，同性恋男性和之前接受过阿奇霉素抗生素治疗的男性患病率较高。它在男性和女性中都会导致尿道炎，在女性还会导致宫颈炎和盆腔炎症。其完整的基因组序列发表于1995年，大小为0.58 Mb，有475个基因，是继流感嗜血杆菌（Heamophilus Influenzae）之后第二个完整测序的细菌基因组。生殖支原体与肺炎支原体（Mycoplasma Pneumoniae）有共同的抗原，在大多数的血清学实验中形成交叉反应。

生殖支原体对培养基要求极高且生长缓慢,培养较难,尤其是从临床标本中培养更不容易成功，这使得检测临床标本中的病原体和随后的分离培养都非常困难。由于没有特异性的血清学检测方法，核酸扩增试验是检测生殖支原体唯一可行的选择。PCR法具有灵敏度高和特异性强的优点，且检测时间短，仅需几个小时即可获得结果。

生殖支原体PCR检测试剂盒选取了粘附素蛋白基因的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向生殖支原体，但同时与流感嗜血杆菌产生一条大小不同且易区分的交叉条带，而与其他生物的基因组无交叉反应。使用本试剂盒检测了16种支原体和17种分布范围或亲缘关系较近的细菌，仅有生殖支原体产生特异性扩增条带，流感嗜血杆菌产生一条较小且易区分的条带。可见本试剂盒具有物种特异性，可用于生殖支原体的鉴定和检测。

 本试剂盒仅供科学研究使用。

 

 

 

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