Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Mycoplasma hominis is a pleomorphic Gram-negative bacterium that can be spherical, filamentous and irregular in shape. The minimum diameter is about 80 to 100 nanometers, the maximum diameter is about 0.5 to 1 micron, and the average diameter About 0.2 to 0.3 microns, it is a common human pathogen found in humans and primates. Mycoplasma hominis mainly lives in the urogenital tract and can also be found in the respiratory tract. It lives a parasitic and saprophytic life in the host and can cause a variety of infections, such as: pelvic inflammatory disease, post-abortion fever, postpartum fever, external genital infections in immunosuppressed people, and meningitis, pneumonia and abscesses in newborns. Mycoplasma hominis has three energy production pathways, including glycolysis, arginine hydrolysis, and riboflavin metabolism.

In vitro culture method is the traditional detection method for Mycoplasma hominis, but this method is relatively Slower, less sensitive, susceptible to contamination by other microorganisms, and sometimes requires tedious microscopic observation. These shortcomings often result in serious waste of time. The PCR method used to detect Mycoplasma hominis has higher sensitivity and better specificity, is not affected by contamination from other microorganisms, and has a short detection time, and the results can be obtained in just a few hours.

The Mycoplasma hominis PCR detection kit selects a sequence of the 16S rRNA gene. PCR identification, and the primers were verified by BLAST to specifically target Mycoplasma hominis and have no cross-reaction with the genomes of other organisms. This kit was used to detect 6 Mycoplasma hominis strains differentially selected based on serotype, genotype and isolated tissue, 20 other mycoplasma species, and two strains each of Gram-negative and Gram-positive bacteria. Only each strain of Mycoplasma hominis produced The amplified bands show that this kit is both species-specific and not affected by intra-species strain variation. This kit is not affected by human genomic DNA and can be used for the identification and detection of Mycoplasma hominis.

This kit is for scientific research only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

人型支原体（Mycoplasma Hominis）是一种多形性革兰氏阴性菌，可呈球形、丝状和不规则形状，最小直径约80至100纳米，最大直径约0.5至1微米，平均直径约0.2至0.3微米，是一种人类常见的病原体，可发现于人类和灵长类动物。人型支原体主要生活在泌尿生殖道，在呼吸道也可发现其存在。它在宿主中营寄生和腐生生活，能引起多种感染，如：盆腔炎症，流产后发热，产后发热，免疫抑制人群的外生殖器感染，以及新生儿的脑膜炎、肺炎和脓肿等。人型支原体有三种能量产生途径，包括糖酵解、精氨酸水解和核黄素代谢。

体外培养法是传统的人型支原体检测方法，但这个方法相对较慢，灵敏度较低，容易受到其他微生物的污染，有时需要繁琐的显微观察。这些缺点往往造成严重的时间浪费。而PCR法用于检测人型支原体，则有更高的灵敏度和更好的特异性，不受其他微生物污染的影响，且检测时间短，仅需几个小时即可获得结果。

人型支原体PCR检测试剂盒选取了16S rRNA基因的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向人型支原体，与其他生物的基因组无交叉反应。使用本试剂盒检测了6个根据血清型、基因型和分离组织差异化选取的人型支原体菌株，20种其他支原体，以及革兰氏阴性和阳性细菌各两株，仅有人型支原体各菌株产生扩增条带，显示本试剂盒既具有物种特异性，又不受种内菌株变异的影响。本试剂盒不受人类基因组DNA的影响，可用于人型支原体的鉴定和检测。

 本试剂盒仅供科学研究使用。

 

 

 

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