Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Haemophilus Mycoplasma can infect many mammals, including cats, dogs, Pigs, cattle, sheep, etc., including humans. There are currently three known mycoplasmas that parasitize in cat blood: Mycoplasma haemofelis, formerly known as Haemobartonella felis), Candidatus Mycoplasma turicensis and Candidatus Mycoplasma haemominutum. These bacteria adhere to and grow on the surface of red blood cells and can also cause some diseases to the host, especially when the cat's immunity is weakened. It has been reported that about 14% of anemic cats are positive for blood mycoplasma.


Mycoplasma felis, also known as Mycoplasma felis and Eperythrocytosis felis, causes The disease ability is stronger than the other two mycoplasmas in cat blood. It parasitizes on the surface of cat red blood cells and is pleomorphic. It can be spherical, ring-shaped or rod-shaped, and can cause infectious hemolytic anemia. Sometimes it has an acute attack and can be fatal; sometimes it has a chronic attack, causing lethargy, anorexia, and mucosal inflammation. Pallor, jaundice, fever, splenomegaly and other symptoms may occur repeatedly. Double or triple infection often occurs with these three mycoplasmas. Mycoplasma felis is often the one with the smallest detected copy number, so a separate detection method is needed to eliminate interference from the other two mycoplasmas.


There are two types of mycoplasma that parasitize in dog blood: Mycoplasma canis and Candidatus Mycoplasma haematoparvum. Mycoplasma canis and Mycoplasma felis are very closely related, and their genome sequences are almost 100% is the same and the shape is similar. Under experimental conditions, cats can be infected with Mycoplasma canis, but dogs cannot be infected with Mycoplasma felis. Mycoplasma canis generally does not cause symptoms and rarely causes obvious hemolysis. In the case of splenectomy or immunosuppression, it may cause some clinical symptoms, including: dehydration, mild anemia, tachycardia, jaundice, die. Mycoplasma canis is the most common hemophilic mycoplasma in dogs and is extremely widely distributed, with a positive rate of approximately 0.5-40%.


Haemophilus Mycoplasma cannot be cultured in vitro, so it is difficult to develop protein detection methods. Commonly used detection methods include microscopy and PCR. The microscopy method has low sensitivity and large experimental errors. It cannot distinguish the types of mycoplasma and is easily interfered by other substances in the blood and artificial smear methods. Mycoplasma can easily fall off the surface of red blood cells, resulting in missed detection. The PCR method has obvious advantages in sensitivity and can distinguish mycoplasma species.


This kit is based on the conserved region of the 16S rRNA gene to design specific primers and The probe can accurately identify Mycoplasma felis and Mycoplasma canis. It only has weak cross-reactivity with Candidatus Mycoplasma haemobos and has no cross-reactivity with other biological genomes. . Candidatus Mycoplasma haemobos is parasitic in cattle blood and will not interfere with the detection of this kit. This kit was used to detect 12 Haemophilus feline Mycoplasma samples, and 4 positive Mycoplasma felis samples were obtained, which was consistent with the sequencing results. It can be seen that this kit can be used for the detection and identification of Haemophilus Felis.


This kit is for scientific research only.

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

嗜血支原体可以感染很多哺乳动物，包括猫、犬、猪、牛、羊等，也包括人类。目前已知寄生于猫血中的支原体有三种：猫血支原体（Mycoplasma haemofelis，旧称Haemobartonella felis），Candidatus Mycoplasma turicensis和Candidatus Mycoplasma haemominutum。这些细菌粘附在红细胞表面生长，也会给宿主造成一些疾病，尤其是在猫免疫力下降的时候。有报道约14%的贫血猫呈血液支原体阳性。

猫血支原体又称猫嗜血支原体、猫附红细胞体，致病能力强于猫血中的另两种支原体。其寄生于猫红细胞表面，具多形性，可呈现为球状、环状或杆状，可引起传染性溶血贫血症，有时呈急性发作，可致命；有时呈慢性发作，出现嗜睡、厌食、粘膜惨白及黄疸、发烧、脾脏肿大等症状，并可能出现反复发作。这三种支原体常发生双重或三重感染，猫血支原体往往是检出拷贝数最少的，因此需要单独的检测方法排除另两种支原体的干扰。

寄生于犬血中的支原体有两种：犬血支原体（Mycoplasma haemocanis ，旧称Haemobartonella canis)和Candidatus Mycoplasma haematoparvum。犬血支原体与猫血支原体亲缘关系非常近，基因组序列几乎100%相同，形态也类似。在实验条件下，猫可以感染犬血支原体，而犬不能感染猫血支原体。犬血支原体一般不引起症状，很少引起明显的溶血，在做了脾切除手术或者发生了免疫抑制的情况下，可能引起一些临床症状，包括：脱水、轻度贫血、心跳过速、黄疸、死亡。犬血支原体是犬类中最常见的嗜血支原体，分布极为广泛，阳性率大约在0.5-40%之间。

嗜血支原体无法在体外培养，因此难以开发蛋白类检测方法。常用的检测方法包括显微镜镜检法和PCR法。镜检法灵敏度低，实验误差大，无法区分支原体种类，易受血液中其他物质以及人工涂片方法的干扰，且支原体易从红细胞表面脱落，造成漏检。而PCR法在灵敏度上则有明显的优势，且可以区分支原体种类。

本试剂盒基于16S rRNA基因的保守区域，设计特异性引物和探针，可以准确识别猫血支原体和犬血支原体，仅与Candidatus Mycoplasma haemobos有较弱的交叉反应，与其他生物基因组没有交叉反应。Candidatus Mycoplasma haemobos寄生于牛血中，不会对本试剂盒的检测产生干扰。使用本试剂盒对12个猫嗜血支原体样本进行检测，获得4个猫血支原体阳性，与测序结果一致，可见本试剂盒可用于猫嗜血支原体的检测和鉴定。

本试剂盒仅供科学研究使用。

 

 

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