Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Mycoplasma Pulmonis is a respiratory pathogen naturally occurring in rats and mice that can cause murine respiratory mycoplasmosis. A pneumonia-like chronic disease that sometimes does not show clinical symptoms and is therefore easily overlooked. Mycoplasma pulmonis is transmitted through close contact between carrier animals, in the form of aerosols through breathing, and through the placenta. The prevalence of M. pulmonis varies widely among rats in different laboratories, with infection rates ranging from 3% to nearly 100%. One study found that about 60% of healthy people acquired infection from M. pulmonis -positive rats. It has been reported that Mycoplasma pulmonis DNA can be detected from the human oropharynx, but the strain has not been successfully isolated and cultured. It is unclear whether M. pulmonis can cause disease in humans.


In vitro culture methods, serological methods and PCR methods can all be used to detect pulmonary mycoplasma . In vitro culture is a classic detection method, but it takes a long time. Serological methods, such as ELISA, are less sensitive, often cross-react with other rodent mycoplasma, and cannot be detected in early stages of infection or in immunodeficient mice. . The PCR method has higher sensitivity and stronger specificity, and the detection time is short. The results generally only take a few hours to obtain, so it has significant advantages.

The Pulmonary Mycoplasma PCR Detection Kit selects a sequence of the 16S rRNA gene for PCR Identified, primers passed BLAST has been verified to specifically target Mycoplasma pulmonis and can clearly distinguish the genomes of other organisms. This kit was used to detect 11 rodent-related mycoplasmas, Staphylococcus aureus, Escherichia coli, and genomic DNA of rats and mice. Only Mycoplasma pulmonis was detected. The strains produced specific amplified bands. Through comparative testing of 7 throat swabs, this kit achieved consistent results with the in vitro culture method, while the ELISA method resulted in false negatives due to insufficient sensitivity. It can be seen that this kit is species-specific and can be used for the identification and detection of pulmonary mycoplasma.

This kit is for scientific research only.

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

肺支原体（Mycoplasma Pulmonis）是一种天然存在于大鼠和小鼠的呼吸道病原体，可引起鼠类呼吸道支原体病，这是一种肺炎样慢性病，有时不会出现临床症状，因此容易被忽视。肺支原体的传播方式为：载体动物之间的密切接触，通过呼吸以气溶胶形式传播，也可通过胎盘传播。在不同实验室的大鼠中肺支原体的流行程度差异很大，感染率可从3％至接近100％。一项研究发现约60%的健康人会从肺支原体阳性大鼠获得感染。有报道从人类口咽部可检测出肺支原体DNA，但没有成功分离培养出菌株。目前还不清楚肺支原体会否引起人类疾病。

体外培养法、血清学方法和PCR法均可用于检测肺支原体。体外培养法是经典的检测方法，但耗时较长，而血清学方法，如ELISA，则灵敏度较低，常与其他啮齿类支原体有交叉反应，并且在感染早期或免疫缺陷鼠中不能进行检测。PCR法则具有更高的灵敏度和更强的特异性，且检测时间短，一般仅需几个小时即可获得结果，因此具备显著的优势。

肺支原体PCR检测试剂盒选取了16S rRNA基因的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向肺支原体，可明显区分其他生物的基因组。使用本试剂盒检测了11种与鼠类相关的支原体、金黄色酿脓葡萄球菌（革兰氏阳性菌）、大肠杆菌（革兰氏阴性菌）、以及大小鼠的基因组DNA，仅有肺支原体菌株产生特异性扩增条带。通过对7个喉拭子的对比检测，本试剂盒与体外培养法取得了一致的结果，而ELISA法则因灵敏度不足而出现假阴性。可见本试剂盒具有物种特异性，可用于肺支原体的鉴定和检测。

 本试剂盒仅供科学研究使用。

 

 

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