Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Mycoplasma Arthritidis, also known as Mycoplasma arthritis, is a Gram-negative facultative anaerobic bacterium. Shape, lack of cell wall. Mycoplasma arthritis is a natural pathogen in rats that causes severe polyarthritis. Arthritis can be experimentally induced in rats by injection of Mycoplasma arthritis through the tail vein. Mice develop the disease more slowly than rats, and the lesions are similar to human rheumatoid arthritis. The toxic factors of Mycoplasma arthritis are the superantigen MAM secreted by it and the adhesion molecule MAA which plays a role in cell adhesion. Most mycoplasmas produce energy through glycolysis, while a few produce energy through other pathways, such as Ureaplasma which produces energy through the decomposition of urea. Mycoplasma arthritis supplies energy through non-glycolytic pathways, using arginine catabolism as the main energy source, and genes required for arginine catabolism have been identified in the genome sequence of Mycoplasma arthritis.


Both in vitro culture methods and PCR methods can be used to detect Mycoplasma arthritis. In vitro culture method is a classic detection method, but it takes a long time. The use of PCR method has higher sensitivity and short detection time, and the results can be obtained in only a few hours.


The Mycoplasma arthritis PCR detection kit selects the characteristic superantigen MAM gene PCR identification was performed, and the primers were verified by BLAST to specifically target Mycoplasma arthritis and otherThere is no cross-reactivity between the genomes of organisms. This kit was used to detect 8 species of mycoplasma and 7 species of bacteria that are closely distributed, as well as 131 joint fluid samples from arthritis patients. Only Mycoplasma arthritis produced specific bands, and 19 samples were identified as Mycoplasma arthritis. Positive. It can be seen that this kit is species-specific and can be used for the identification and detection of Mycoplasma arthritis.


This kit is for scientific research only.

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

关节炎支原体（Mycoplasma Arthritidis），或称关节炎霉浆菌，是一种革兰氏阴性兼性厌氧细菌，呈多形性，缺乏细胞壁。关节炎支原体是大鼠的天然病原体，引起严重的多发性关节炎。通过尾静脉注射关节炎支原体可以实验性诱导大鼠关节炎。小鼠发病比大鼠慢，病变与人类风湿性关节炎相似。关节炎支原体产生毒性的因素是其分泌的超抗原MAM和起细胞粘附作用的粘附分子MAA。大多数支原体通过糖酵解产生能量，少数通过其他途径，如解脲支原体（Ureaplasma）通过分解尿素产生能量。关节炎支原体通过非糖酵解途径供能，以精氨酸分解代谢为主要的能量来源，并且在关节炎支原体的基因组序列中鉴定出精氨酸分解代谢所需的基因。

体外培养法和PCR法均可用于检测关节炎支原体。体外培养法是经典的检测方法，但耗时较长，而使用PCR法则有更高的灵敏度，且检测时间短，仅需几个小时即可获得结果。

关节炎支原体PCR检测试剂盒选取了特征性的超抗原MAM基因进行PCR鉴定，引物经BLAST验证为特异性靶向关节炎支原体，与其他生物的基因组无交叉反应。使用本试剂盒检测了分布范围较近的8种支原体和7种细菌，以及131份关节炎患者的关节液样本，仅有关节炎支原体产生特异性条带，有19份样本鉴定为关节炎支原体阳性。可见本试剂盒具有物种特异性，可用于关节炎支原体的鉴定和检测。

 本试剂盒仅供科学研究使用。

 

 

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