Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of turkey mycoplasma, no cross-reaction with other biological genomes.

2. High sensitivity, the lowest detection limit is 10 copies in the reaction solution.

3. Use hot-start Taq enzyme to inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5, with dUTP and UDG enzymes, which can reduce the contamination of residual DNA.

 

Mycoplasma turkey is a turkey-specific pathogen that can be transmitted through eggs or by ticks. It is distributed around the world and is prone to infection in concentrated breeding environments. It usually affects young poultry under 16 weeks of age. The incidence of airsacculitis associated with Mycoplasma in turkeys is high in naturally infected culled poultry. Mycoplasma turkeys can be isolated from the upper respiratory tract, air sacs, penis, semen, cloaca, and sinuses. The lesions caused are mainly air sacculitis in offspring turkeys, but others include sinusitis, low hatchability, skeletal deformities, crooked neck disease, and growth spurts. Developmental delay, etc.


Mycoplasma turkey can be cultured in mycoplasma agar and broth containing arginine When cultured on a substrate, it may take up to 7 days for colonies to appear and eventually develop a typical fried egg appearance. Serological tests including growth inhibition, metabolic inhibition, and immunofluorescence can also be used to identify M. turkeys, with immunofluorescence being effective even in mixed cultures. Due to antigenic variation among strains of Mycoplasma turkeyThe differences are large, so serological methods often encounter difficulties. The PCR method has the characteristics of short detection time and high sensitivity, and has become the most commonly used detection method for avian mycoplasma. The results can be obtained in just a few hours without the need for a long culture process. Ordinary PCR experiments are more cumbersome and can only conduct qualitative analysis of target DNA; in contrast, real-time quantitative PCR can not only conduct quantitative analysis of target DNA, but the experimental steps are also simpler, more sensitive, and less susceptible to environmental contamination. Influence.

 

Probe method Mycoplasma turkey quantitative PCR kit selects 16-23S rRNA gene The interval sequence is used as a target and has been verified by BLAST to specifically target Mycoplasma turkey and has no cross-reactivity with the genomes of other organisms. This kit was used to detect 17 species of avian mycoplasma, and no cross-reaction occurred. Ten different strains of M. turkey were tested and all were positive.

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测火鸡支原体，与其他生物基因组无交叉反应。

2， 灵敏度高，最低检测极限为反应液中10个拷贝。

3， 使用热启动的Taq酶，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有dUTP和UDG酶，可降低残留DNA的污染。

 

火鸡支原体（Mycoplasma Meleagridis）是火鸡特异性的病原体，可经蛋传递或由蜱虫传播，分布于全球，在集中饲养环境中容易出现感染，通常影响16周龄以下的幼禽。在自然感染淘汰的幼禽中，与火鸡支原体有关的气囊炎的发生率很高（约20-65%）。火鸡支原体可以从上呼吸道、气囊、阴茎、精液、泄殖腔和鼻窦分离出来，引起的病变主要是后代火鸡的气囊炎，其它还包括鼻窦炎、低孵化率、骨骼畸形、歪脖病和生长发育迟缓等。

火鸡支原体可以在含有精氨酸的支原体琼脂和肉汤培养基上培养，菌落可能需要7天才能出现，并最终形成一个典型的煎蛋外观。也可以使用包括生长抑制、代谢抑制和免疫荧光在内的血清学测试方法来鉴定火鸡支原体，其中免疫荧光即使在混合培养物中也是有效的。由于火鸡支原体的菌株之间抗原变异较大，因此血清学方法常常遇到困难。PCR法具有检测时间短、灵敏度高的特点，成为目前使用较多的鸟类支原体检测方法，只需数个小时即可获得结果，无需漫长的培养过程。普通PCR实验操作较为繁琐，只能对目标DNA进行定性分析；相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高，更少受环境污染的影响。

探针法火鸡支原体定量PCR试剂盒选取16-23S rRNA基因区间序列作为靶点，经BLAST验证，特异性靶向火鸡支原体，与其他生物基因组无交叉反应。使用本试剂盒检测了17种鸟类支原体，均无交叉反应发生。检测十个不同的火鸡支原体菌株，均为阳性。

 

 

 

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