Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma Iowa without cross-reaction with other biological genomes.

2. The sensitivity is extremely high, with the lowest detection limit being 10 copies in the reaction solution.

3. Use hot-start Taq enzyme to inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5, with dUTP and UDG enzymes, which can reduce the contamination of residual DNA.

Mycoplasma Iowa is an avian mycoplasma that can be isolated from many birds and its spread can cause significant economic losses to the turkey farming industry. Compared with other avian mycoplasmas, it has some different properties, such as: resistance to bile salts, prone to occur in the gastrointestinal tract, strong resistance to many antibacterial agents, and a very large degree of antigenic variation between strains. Common manifestations of natural infection with M. Iowa are death of turkey embryos in late hatching, a 2-5% reduction in hatchability, and leg abnormalities in chicks. In experimental infections, some strains can cause embryonic death in chicks and turkeys and, depending on the route of infection and the age of the bird, joint and skeletal abnormalities, growth retardation, poor feather development, and occasionally air sacs inflammation. In turkeys it is primarily transmitted through eggs, but can also be transmitted laterally. The fallopian tubes, semen, and cloaca of mature birds are sources of infection.


The antigenic variability of Mycoplasma Iowa is large, and chickens and turkeys are resistant to it The humoral response is weak and therefore not suitable for testing using serological methodsMeasurement. In vitro culture method is a classic detection method, but it is time-consuming. The PCR method has the characteristics of short detection time, high sensitivity and strong specificity, and has become the most commonly used detection method for avian mycoplasma. The results can be obtained in just a few hours without the need for a long culture process. Ordinary PCR experiments are more cumbersome and can only conduct qualitative analysis of target DNA; in contrast, real-time quantitative PCR can not only conduct quantitative analysis of target DNA, but the experimental steps are also simpler, more sensitive, and less susceptible to environmental contamination. Influence.


The probe Mycoplasma Iowa quantitative PCR kit selects the upstream of the 16S rRNA gene A specific sequence is used as a target. It has been verified by BLAST that it specifically targets Mycoplasma Iowa and has no cross-reactivity with other biological genomes. This kit was used to detect 17 species of avian mycoplasma, and no cross-reaction occurred. Ten different strains of Mycoplasma Iowa were tested and all were positive. It can be seen that this kit is specific for Mycoplasma Iowa and is not interfered by intraspecies variation.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测衣阿华支原体，与其他生物基因组无交叉反应。

2， 灵敏度极高，最低检测极限为反应液中10个拷贝。

3， 使用热启动的Taq酶，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有dUTP和UDG酶，可降低残留DNA的污染。

 

衣阿华支原体（Mycoplasma Iowae）是一种鸟类支原体，可以从许多鸟类中分离出来，其传播能给火鸡养殖业造成明显经济损失。它与其他鸟类支原体相比具有一些不同的性质，如：耐胆盐，好发于胃肠道，对许多抗菌剂的抗性较强，菌株之间的抗原变异程度非常大等。衣阿华支原体自然感染的常见表现是孵化晚期火鸡胚胎的死亡，孵化率降低2-5%，以及仔鸡腿部异常。在实验性感染中，一些菌株可引起小鸡和火鸡胚胎死亡，根据感染的路线和鸟的年龄的不同，还可以引起关节和骨骼异常，生长迟缓，羽毛发育不佳，以及偶尔发生的气囊炎。在火鸡中它主要是靠卵子传播，也可以进行横向传播。成熟鸟类的输卵管、精液和泄殖腔是感染的来源。

衣阿华支原体的抗原变异性大，鸡和火鸡对其体液反应很弱，因此不适合使用血清学方法进行检测。体外培养法是经典的检测方法，但耗时较长。PCR法具有检测时间短、灵敏度高、特异性强的特点，成为目前使用较多的鸟类支原体检测方法，只需数个小时即可获得结果，无需漫长的培养过程。普通PCR实验操作较为繁琐，只能对目标DNA进行定性分析；相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高，更少受环境污染的影响。

探针法衣阿华支原体定量PCR试剂盒选取16S rRNA基因上游的一段特异性序列作为靶点，经BLAST验证，特异性靶向衣阿华支原体，与其他生物基因组无交叉反应。使用本试剂盒检测了17种鸟类支原体，均无交叉反应发生。检测十个不同的衣阿华支原体菌株，均为阳性。可见本试剂盒对衣阿华支原体具有特异性，且不受种内变异的干扰。

 

 

 

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