Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma synoviae without cross-reaction with other biological genomes.

2. High sensitivity, with detection limit below 10 copies per reaction.

3. The DNA polymerase used has the characteristics of strong anti-inhibition ability and good thermal stability; using hot start method, it can Inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5, with dUTP and UDG enzymes, which can reduce the contamination of residual DNA.

Mycoplasma synoviae, also known as Mycoplasma synoviae, is a polymorphic spheroid, slightly smaller than Mycoplasma gallisepticum, and is Gram-negative. There is only one serotype, and the pathogenicity of different strains is different, and the symptoms caused are also different depending on the tropism of the pathogen. Mycoplasma synoviae mainly infects chickens, turkeys and guinea fowl, and mainly young chicks. Ducks, geese, pigeons, Japanese quails, red-legged partridges, etc. can also be infected. Infection can cause acute or chronic systemic diseases, and lead to infectious exudative synovitis, tenosynovitis and bursitis in the synovial membrane and tendon sheaths of joints. Subclinical upper respiratory symptoms are often present and, if combined with Newcastle disease or bacterial infection, can lead to airsacculitis.


The traditional method for detecting Mycoplasma synoviae is in vitro culture. Due to slow growth, sometimes It takes up to three weeks, so the cultures are often contaminated with other mycoplasma or bacteria or fungi. Serological methods are widely used forThe disadvantage of the detection of Mycoplasma synoviae is that it cannot detect early infection. The production of antibodies in the serum lags behind the infection. The serum plate agglutination test can only be performed after at least one week, and the hemagglutination inhibition test can only be performed within three weeks. Therefore, it is not suitable for seroconversion. monitor. The PCR method has the characteristics of short detection time and high sensitivity, and has become the most commonly used detection method for avian mycoplasma. The results can be obtained in just a few hours without the need for a long culture process. Ordinary PCR experiments are more cumbersome and can only conduct qualitative analysis of target DNA; in contrast, real-time quantitative PCR can not only conduct quantitative analysis of target DNA, but the experimental steps are also simpler, more sensitive, and less susceptible to environmental contamination. Influence.


Probe method Mycoplasma synoviae quantitative PCR kit selects 16-23S rRNA gene The interval sequence is used as a target and has been verified by BLAST to specifically target Mycoplasma synoviae and has no cross-reactivity with other biological genomes. This kit was used to detect 17 species of avian mycoplasma, and no cross-reaction occurred. Ten different strains of M. synoviae were tested and all were positive.

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测滑液支原体，与其他生物基因组没有交叉反应。

2， 灵敏度高，检测极限低于每反应10个拷贝。

3， 使用的DNA聚合酶具有抗抑制能力强和热稳定性好的特点；采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有dUTP和UDG酶，可降低残留DNA的污染。

 

滑液支原体（Mycoplasma Synoviae），也称为滑液囊支原体，呈多形态的球形体(直径约0．2～0．4微米)，比鸡毒支原体稍小，革兰氏染色阴性，只有一个血清型，不同菌株的致病力有差异，引起的症状也因病原的趋向性而不同。滑液支原体主要感染鸡、火鸡以及珍珠鸡，且以幼雏为主，鸭、鹅、鸽、日本鹌鹑、红腿鹧鸪等也可感染。感染后可引起急性或慢性的全身性疾病，并导致关节的滑液囊膜及腱鞘等发生传染性渗出性滑膜炎、腱鞘滑膜炎及黏液囊炎。常出现亚临床性上呼吸道症状，如与新城疫或细菌感染相结合，可导致气囊炎。

检测滑液支原体的传统方法是体外培养，由于生长缓慢，有时需长达三周的时间，因此培养物常被其他支原体或细菌、真菌污染。血清学方法广泛用于对滑液支原体的检测，其缺点是不能检测早期感染，血清中抗体的产生滞后于感染，至少一周后才能进行血清平皿凝集实验，三周内才可以进行血凝抑制实验，因此不适用于血清转化监测。而PCR法具有检测时间短、灵敏度高的特点，成为目前使用较多的鸟类支原体检测方法，只需数个小时即可获得结果，无需漫长的培养过程。普通PCR实验操作较为繁琐，只能对目标DNA进行定性分析；相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高，更少受环境污染的影响。

探针法滑液支原体定量PCR试剂盒选取16-23S rRNA基因区间序列作为靶点，经BLAST验证，特异性靶向滑液支原体，与其他生物基因组无交叉反应。使用本试剂盒检测了17种鸟类支原体，均无交叉反应发生。检测十个不同的滑液支原体菌株，均为阳性。

 

 

 

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