Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma gallisepticum without cross-reaction with other biological genomes.

2. The sensitivity is extremely high, and the lowest detection limit is 1 copy in the reaction solution.

3. The DNA polymerase used has the characteristics of strong anti-inhibition ability and good thermal stability; using hot start method, it can Inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5. With UDG enzyme and dUTP, it can reduce the contamination of residual DNA.

Mycoplasma gallisepticum, also known as Mycoplasma gallisepticum, Mycoplasma gallisepticum, and Mycoplasma gallisepticum, is an important poultry pathogenic mycoplasma that often causes significant economic losses and sometimes can lead to entire Groups of poultry were culled. It is the causative agent of chronic respiratory diseases and can cause upper respiratory tract infection, showing different symptoms such as rales, cough, runny nose, conjunctivitis, sinusitis, reduced egg production, increased embryonic mortality, and can cause air sacs in severe cases. inflammation, it has also been reported that it can cause slow weight gain and meat loss in broiler chickens, as well as cause infectious infertility through eggs. Although M. gallisepticum infections may be asymptomatic, they can make birds more susceptible to secondary infections with bacteria such as E. coli, and viruses such as Newcastle disease, infectious bronchitis, and infectious laryngotracheitis. Mycoplasma gallisepticum can be transmitted through aerosols, food, water and various items in the environment. In many cases, the source of transmission cannot be identified. Mycoplasma gallisepticum is also the causative agent of infectious sinusitis in birds of all ages, including turkeys, ducks, geese, wild birds, pigeons, and peacocks.


Mycoplasma gallisepticum can be cultured in vitro, but it grows slowly and has high nutritional requirements. It often takes one to several weeks to complete growth and serological identification. .Due to the long time, its growth is sometimes masked by other faster-growing upper respiratory tract saprophytic mycoplasmas. Serological methods are often used to detect Mycoplasma gallisepticum, but it has an important shortcoming that the detection lags behind the infection. After infection, The plate rapid agglutination test can be performed for at least one week, while the red blood cell agglutination inhibition test must be performed within three weeks after infection; another disadvantage is that the serological method is not specific enough, often cross-reacts with other mycoplasmas, and may also be sensitive to different strains. Difference. The PCR method has the characteristics of short detection time, high sensitivity and strong specificity, and has become the most commonly used detection method for avian mycoplasma. The results can be obtained in just a few hours without the need for a long culture process. Ordinary PCR experimental operations It is more cumbersome and can only conduct qualitative analysis of target DNA; in contrast, real-time quantitative PCR can not only conduct quantitative analysis of target DNA, but also has simpler experimental steps, higher sensitivity, and is less affected by environmental pollution.


The probe method Mycoplasma gallisepticum quantitative PCR kit selects the cytoadhesin protein gene as The target, verified by BLAST, specifically targets Mycoplasma gallisepticum and has no cross-reaction with other biological genomes. This kit was used to detect 17 species of avian Mycoplasma, and no cross-reaction occurred. Detected ten different Mycoplasma gallisepticum strains. , all positive.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测鸡毒支原体，与其他生物基因组无交叉反应。

2， 灵敏度极高，最低检测极限为反应液中1个拷贝。

3， 使用的DNA聚合酶具有抗抑制能力强和热稳定性好的特点；采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

鸡毒支原体（Mycoplasma Gallisepticum），又称为鸡败血支原体、鸡毒霉形体、鸡败血霉形体，是一种重要的家禽致病性支原体，常造成重大经济损失，有时能导致整群家禽被淘汰。它是慢性呼吸系统疾病的病原体，可导致上呼吸道感染，表现出啰音、咳嗽、流鼻涕、结膜炎、鼻窦炎、产蛋量减少、胚胎死亡率上升等不同的症状，严重时可引起气囊炎，还有报道其能使肉鸡体重增加缓慢和肉品下降，以及经蛋引起感染性不育。尽管鸡毒支原体感染可能没有临床症状，但它们可以使禽类更容易继发感染大肠杆菌等细菌，以及新城疫、传染性支气管炎和传染性喉气管炎等病毒。鸡毒支原体可以通过气溶胶、食物、水和环境中的各种物品进行传播，很多时候无法查明传播源头。鸡毒支原体也是火鸡、鸭、鹅、野鸟、鸽子和孔雀等鸟类各年龄段的传染性鼻窦炎的病原体。

鸡毒支原体可进行体外培养，但生长缓慢，对营养要求比较高，常需要一到数个星期才能完成生长和血清学鉴定工作。由于时间较长，其生长有时会被其它生长较快的上呼吸道腐生支原体所掩盖。血清学方法常用于鸡毒支原体的检测，但其有个重要的缺点就是检测滞后于感染，在感染后至少一周才能进行平板快速凝集实验，而红血球凝聚抑制实验则必须在感染后三周以内进行；另一个缺点是血清学方法特异性不足，常与其他支原体发生交叉反应，对不同菌株也可能有灵敏度差异。PCR法具有检测时间短、灵敏度高、特异性强的特点，成为目前使用较多的鸟类支原体检测方法，只需数个小时即可获得结果，无需漫长的培养过程。普通PCR实验操作较为繁琐，只能对目标DNA进行定性分析；相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高，更少受环境污染的影响。

探针法鸡毒支原体定量PCR试剂盒选取细胞粘附素蛋白基因作为靶点，经BLAST验证，特异性靶向鸡毒支原体，与其他生物基因组无交叉反应。使用本试剂盒检测了17种鸟类支原体，均无交叉反应发生。检测十个不同的鸡毒支原体菌株，均为阳性。

 

 

 

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