Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

Mycoplasma cloacale, also translated as Mycoplasma cloacae, belongs to the family Mycoplasma >Mycoplasmataceae) is a Gram-negative bacterium that was originally found in the cloaca of turkeys and can also be isolated from ducks, geese and other birds. The main symptom after infection is a decrease in hatching success rate, thus result in economic losses. Mycoplasma cloaca can be transmitted vertically through eggs.

Mycoplasma cloaca can be detected through culture. The disadvantage is that culture is more difficult and the appearance It has a typical omelette shape and is difficult to distinguish from other mycoplasmas, so biochemical identification is required. Serological methods are time-consuming and require purified in vitro culture colonies, and antiserum is not easy to obtain. Using the PCR method has the advantages of high sensitivity and short detection time, and results can be obtained in just a few hours.

Mycoplasma cloaca PCR detection kit selects DNA polymerase gene for PCR identification, primers It has been verified by BLAST that it specifically targets Mycoplasma cloaca and has no cross-reactivity with the genomes of other organisms. This kit was used to detect 19 Mycoplasma cloaca strains and 15 other avian Mycoplasma species, and only 19 Mycoplasma cloaca strains produced specific bands; 28 waterfowl samples were tested, and 18 were positive, which was consistent with the sequencing results. . It can be seen that this kit is species-specific and can be used for the identification and detection of Mycoplasma cloaca.

 

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

泄殖腔支原体（Mycoplasma cloacale），也有译作阴沟支原体，属于柔膜菌纲（Mollicutes）支原体科（Mycoplasmataceae），是一种革兰氏阴性菌，最初发现于火鸡泄殖腔，也可从鸭、鹅及其他鸟类中分离出来，感染后的主要症状是孵卵成功率下降，从而导致经济损失。泄殖腔支原体可通过卵进行垂直传播。

泄殖腔支原体可通过培养法进行检测，缺点是培养较为困难，外观为典型的煎蛋状，与其他支原体不易区分，需进行生化鉴定。血清学方法则耗时较长，需要纯化的体外培养菌落，抗血清也不易获得。使用PCR法则具有高灵敏度的优点，且检测时间短，仅需几个小时即可获得结果。

泄殖腔支原体PCR检测试剂盒选取DNA聚合酶基因进行PCR鉴定，引物经BLAST验证为特异性靶向泄殖腔支原体，与其他生物的基因组无交叉反应。使用本试剂盒检测了19个泄殖腔支原体菌株以及15种其他鸟类支原体，仅有19个泄殖腔支原体菌株产生特异性条带；对28个水禽类样本进行检测，获得18个阳性，同测序结果一致。可见本试剂盒具有物种特异性，可用于泄殖腔支原体的鉴定和检测。

 

 

 

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