Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma villoni. When preparing samples, be careful not to contaminate other animal blood products.

2. High sensitivity, the detection rate is 58% when there is only one genome in the reaction solution. The linear range spans 8 orders of magnitude.

3. The DNA polymerase used has the characteristics of strong anti-inhibition ability and good thermal stability; using hot start method, it can Inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5, with dUTP and UDG enzymes, which can reduce the contamination of residual DNA.

Haemophilic Mycoplasma is a type of bacteria that lives on the surface of red blood cells. It has no cell wall and can infect many mammals, including cats, dogs, pigs, cattle, sheep, etc. Including humans. Haemophilus Mycoplasma was once named Hemobartonella and Eperythrozoon . Based on 16S rRNA gene sequence analysis, it was reclassified as a mycoplasma. . There are two types of haemophilic mycoplasmas that have been found in cattle: Mycoplasma wenyonii and Candidatus Mycoplasma haemobos.


DimensionMycoplasma wenyonii, formerly known as Eperythrozoon wenyonii, is also known as Mycoplasma wenyi, Mycoplasma wenyoni, Eperythrozoon wenyi, Eperythrozoon wenyoni, Eperythrozoon bovis. , Haematoma wendii and Hemoglobin wenschlii were first discovered in 1934 and have only been found in cattle so far. Blood smear test results show that it is free in plasma or attached to the surface of red blood cells. It is widely distributed around the world and can be associated with Anaplasma, Babesia and Theileria form a co-infection. In most cases, cattle have no clinical manifestations after infection, and sometimes symptoms such as anemia, fever, hind limb edema, nipple swelling, sudden drop in milk production, and weight loss occur. It is difficult to completely remove the infection and may carry the bacteria for life. Blood-sucking arthropods are generally believed to be the route of transmission.


Mycoplasma villoni has no suitable in vitro culture method, so it is difficult to develop proteins Class detection method. The detection method generally uses the Giemsa-stained blood smear method. The detection sensitivity and specificity of the blood smear method are relatively poor, and it is easily interfered by artificial preparation methods and other components in the blood, and mycoplasma can easily fall off the surface of red blood cells, resulting in missed detection. The PCR method has obvious advantages in sensitivity and can distinguish mycoplasma species. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.


This kit designs specific primers and probes based on the 16S rRNA gene. Accurately identify Mycoplasma villoni, together with Mycoplasma ovis, Mycoplasma suis, and Candidatus Mycoplasma that parasitizes sika deer haemocervae has cross-reactivity with Candidatus Mycoplasma erythrocervae, but has no cross-reactivity with the genomes of Candidatus Mycoplasma haemobos and other organisms. Since Haemophilus Mycoplasma has strict host specificity, you only need to pay attention to strict operation during detection and do not contaminate the blood source of other animals, which will not affect the detection. for different healthA total of 133 positive results were obtained from 159 cattle with fatal anemia, 33 positive results from 57 cattle with unrelated diseases, 38 positive results from 61 healthy cattle, and 47 positive results from healthy cattle. Only 2 positives were obtained in calves.


This kit contains hot-start DNA polymerase, dNTP, primers, probes, positive controls, ROX dye, and UDG to prevent residual DNA contamination. Users only need to prepare DNA samples and use them.


The DNA polymerase used in this kit is derived from extremothermophilic bacteria, which has been modified to have stronger resistance to inhibition and thermal stability than ordinary Taq enzymes. By binding specific monoclonal antibodies, DNA polymerase activity is inhibited at room temperature. During the thermal denaturation phase of the PCR cycle, the antibodies are heated and removed, and the DNA polymerase activity is restored at this time, thus obtaining the "hot start" function. Hot start can reduce the contamination of residual DNA and improve PCR amplification efficiency, sensitivity and target fragment yield.


UDG and dUTP are used to eliminate residual contamination in PCR amplification. dUTP ensures that amplified DNA contains uracil. UDG removes uracil residues from single- or double-stranded DNA, thereby preventing uracil-containing DNA from being used as a template for further amplification. UDG is inactivated by high temperature during normal PCR cycles and has no effect on the PCR reaction.

 

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测维容氏支原体，准备样品时注意不要污染其他动物血制品。

2， 灵敏度高，反应液中仅1个基因组时检出率为58%。线性范围跨越8个数量级。

3， 使用的DNA聚合酶具有抗抑制能力强和热稳定性好的特点；采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有dUTP和UDG酶，可降低残留DNA的污染。

 

嗜血支原体（Hemotropic Mycoplasma，或hemoplasma)是一类寄生于红细胞表面的细菌，无细胞壁，可以感染很多哺乳动物，包括猫、犬、猪、牛、羊等，也包括人类。嗜血支原体曾被命名为血巴通氏体（Hemobartonella）和附红细胞体（Eperythrozoon），根据16S rRNA基因序列分析，被重新归类为支原体。在牛体内已发现的嗜血支原体有两种：维容氏支原体（Mycoplasma wenyonii）和Candidatus Mycoplasma haemobos。

维容氏支原体，旧称维容氏附红细胞体（Eperythrozoon wenyonii），也有称为温氏支原体、文氏支原体、温氏附红细胞体、维氏附红细胞体、牛附红细胞体、温氏血虫体、文氏附红血球体，首次发现于1934年，目前为止仅在牛体内发现。血涂片检测结果显示，其游离于血浆或附着在红细胞表面，在全世界广泛分布，可与无形体（Anaplasma）、巴贝虫（Babesia）和泰勒虫（Theileria）形成共感染。多数情况下牛在感染后无临床表现，有时会出现贫血、发热、后肢水肿、乳头肿胀、产奶量突降和体重减轻等症状。感染后很难完全清除，可能终身带菌。一般认为吸血性节肢动物是其传播途径。

维容氏支原体尚无合适的体外培养方法，因此难以开发蛋白类检测方法。检测方法一般采用吉姆萨染色的血涂片法。血涂片法的检测灵敏度和特异性均比较差，容易受到人工制片方法和血液里其他成分（如：Howell–Jolly体）的干扰，且支原体易从红细胞表面脱落，造成漏检。而PCR法在灵敏度上则有明显的优势，且可以区分支原体种类。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒基于16S rRNA基因设计特异性引物和探针，可以准确识别维容氏支原体，与羊支原体（Mycoplasma ovis）、猪支原体（Mycoplasma suis）、以及寄生于梅花鹿的Candidatus Mycoplasma haemocervae和Candidatus Mycoplasma erythrocervae有交叉反应，而与Candidatus Mycoplasma haemobos及其他生物的基因组无交叉反应。由于嗜血支原体有严格的宿主特异性，检测时只需注意严格操作，不污染其他动物的血源，即不会对检测造成影响。对不同健康状况的牛群进行检测，在159只患致命贫血牛中获得133个阳性，在57只患不相关疾病牛中获得33个阳性，在61只健康牛中获得38个阳性，而在47只健康牛犊中只获得2个阳性。

本试剂盒包含热启动DNA聚合酶、dNTP（以dUTP代替dTTP）、引物、探针、阳性对照品、ROX染料，和用以防止残余DNA污染的UDG（Uracil- DNA Glycosylase，尿嘧啶-DNA-糖基酶），用户只需准备好DNA样品即可使用。

本试剂盒采用的DNA聚合酶源自极端嗜热菌（Thermus thermophilus），经改造后较普通的Taq酶具有更强的抗抑制能力和热稳定性。通过结合特异性单克隆抗体，在室温下DNA聚合酶活性被抑制。在PCR循环的热变性阶段，抗体受热被去除，此时DNA聚合酶活性恢复，因此获得“热启动”功能。热启动可减少残余DNA的污染，提高PCR扩增效率、灵敏度和目的片段产量。

UDG和dUTP用于消除PCR扩增中的残余污染。dUTP可以确保扩增后的DNA中均含有尿嘧啶。UDG可从单链或双链DNA上去除尿嘧啶残基，从而阻止含有尿嘧啶的DNA作为模板继续扩增。UDG在正常的PCR循环过程中被高温灭活，对PCR反应没有影响。

 

 

 

Cartpieces

• 
• 1

  1Delete

• 
• 6

  6Delete

• 
• q

  qDelete

• 
• P

  PDelete

• 
• +

  +Delete

• 
• 7

  7Delete

• 
• 2

  2Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• P

  PDelete

• 
• �

  �Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• P

  PDelete

• 
• 5

  5Delete

• 
• P

  PDelete

Totalgoods,subtotals:￥Checkout