Details
Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.
Kit Features:
1. This kit can specifically detect Candidatus Mycoplasma haemobos and is not affected by other genomes. interference.
2. This kit has high sensitivity, and the detection rate is 69% when there is only one genome in the reaction solution. The linear range spans 8 orders of magnitude.
3. The DNA polymerase used in this kit has the characteristics of strong resistance to inhibition and good thermal stability; it adopts hot start This method can inhibit non-specific amplification and reduce background fluorescence.
4. This kit comes with a positive control sample, which can be used to test the effectiveness of the kit.
5. With UDG enzyme and dUTP, it can reduce the contamination of residual DNA.
Haemophilic Mycoplasma is a type of bacteria that lives on the surface of red blood cells. It has no cell wall and can infect many mammals, including cats, dogs, pigs, cattle, sheep, etc. Including humans. Haemophilus Mycoplasma was once named Hemobartonella and Eperythrozoon . Based on 16S rRNA gene sequence analysis, it was reclassified as a mycoplasma. . There are two types of haemophilic mycoplasmas that have been found in cattle: Mycoplasma wenyonii and Candidatus Mycoplasma haemobos.
Candidatus Mycoplasma haemobos was discovered relatively late and was not reported until this century. It is widely distributed in China, Japan, and Switzerland. It has been found in , Germany and Brazil, etc., but the clinical symptoms are still unclear. Its 16S rRNA gene is shorter than that of Mycoplasma villonii, with only 81.2% similarity, and 95% similarity with Mycoplasma felis, so it is classified with Mycoplasma felis Mycoplasma is closer.
Candidatus Mycoplasma haemobos is still There is no suitable in vitro culture method, so it is difficult to develop protein detection methods. The detection method generally uses the Giemsa-stained blood smear method. The detection sensitivity and specificity of the blood smear method are relatively poor, and it is susceptible to manual preparation methods and blood There is interference from other components in it, and mycoplasma is easy to fall off from the surface of red blood cells, resulting in missed detection. The PCR method has obvious advantages in sensitivity and can distinguish types of mycoplasma. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, Moreover, the operation is more convenient and less affected by environmental pollution.
This reagent The kit is based on the conserved region of the 16S rRNA gene and is designed with specific primers and probes that can accurately identify Candidatus Mycoplasma haemobos without cross-reaction with other biological genomes. After testing, this kit is compatible with Mycoplasma villoni , 3 types of haemophilic mycoplasma from cats and dogs, and 4 types of non-haemophilic mycoplasma have no cross-reactivity. The kit uses bovine mitochondrial cytochrome gene as an internal reference, which can eliminate PCR inhibitors and DNA extraction failures that cause false negatives.
This kit contains hot-start DNA polymerase, dNTP, Primers, probes, positive controls, ROX dye, and UDG to prevent residual DNA contamination, users only need to prepare DNA samples to use.
The DNA polymerase used in this kit is derived from the extremely thermophilic bacterium and has been modified to have stronger potency than the ordinary Taq enzyme. Resistance to inhibition and thermal stability. By binding specific monoclonal antibodies, DNA polymerase activity is inhibited at room temperature. During the thermal denaturation phase of the PCR cycle, the antibodies are heated and removed, and the DNA polymerase activity is restored at this time, thus obtaining the "hot start" function. Hot start can reduce the contamination of residual DNA and improve PCR amplification efficiency, sensitivity and target fragment yield.
-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。
试剂盒特点：
1， 本试剂盒可以特异性检测Candidatus Mycoplasma haemobos，不受其他基因组干扰。
2， 本试剂盒灵敏度高，反应液中仅1个基因组时检出率为69%。线性范围跨越8个数量级。
3， 本试剂盒使用的DNA聚合酶具有抗抑制能力强和热稳定性好的特点；采用热启动方式，可抑制非特异性扩增，降低背景荧光。
4， 本试剂盒带有阳性对照样品（组分C），可用于检验试剂盒有效性。
5， 带有UDG酶和dUTP，可降低残留DNA的污染。
嗜血支原体（Hemotropic Mycoplasma，或hemoplasma)是一类寄生于红细胞表面的细菌，无细胞壁，可以感染很多哺乳动物，包括猫、犬、猪、牛、羊等，也包括人类。嗜血支原体曾被命名为血巴通氏体（Hemobartonella）和附红细胞体（Eperythrozoon），根据16S rRNA基因序列分析，被重新归类为支原体。在牛体内已发现的嗜血支原体有两种：维容氏支原体（Mycoplasma wenyonii）和Candidatus Mycoplasma haemobos。
Candidatus Mycoplasma haemobos发现较晚，直至本世纪才有报导，分布较为广泛，在中国、日本、瑞士、德国和巴西等都有发现，临床症状尚不清楚。其16S rRNA基因较维容氏支原体短，相似度仅81.2%，与猫血支原体有95%的相似度，因而在分类上与猫血支原体更为接近。
Candidatus Mycoplasma haemobos尚无合适的体外培养方法，因此难以开发蛋白类检测方法。检测方法一般采用吉姆萨染色的血涂片法。血涂片法的检测灵敏度和特异性均比较差，容易受到人工制片方法和血液里其他成分的干扰，且支原体易从红细胞表面脱落，造成漏检。而PCR法在灵敏度上则有明显的优势，且可以区分支原体种类。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。
本试剂盒基于16S rRNA基因的保守区域，设计特异性引物和探针，可以准确识别Candidatus Mycoplasma haemobos，与其他生物基因组无交叉反应。经检测，本试剂盒与维容氏支原体、猫和犬的3种嗜血支原体、及4种非嗜血支原体均无交叉反应。试剂盒以牛线粒体细胞色素基因作为内参，可以排除引起假阴性的PCR抑制剂和DNA提取失败。
本试剂盒包含热启动DNA聚合酶、dNTP（以dUTP代替dTTP）、引物、探针、阳性对照品、ROX染料，和用以防止残余DNA污染的UDG（Uracil- DNA Glycosylase，尿嘧啶-DNA-糖基酶），用户只需准备好DNA样品即可使用。
本试剂盒采用的DNA聚合酶源自极端嗜热菌（Thermus thermophilus），经改造后较普通的Taq酶具有更强的抗抑制能力和热稳定性。通过结合特异性单克隆抗体，在室温下DNA聚合酶活性被抑制。在PCR循环的热变性阶段，抗体受热被去除，此时DNA聚合酶活性恢复，因此获得“热启动”功能。热启动可减少残余DNA的污染，提高PCR扩增效率、灵敏度和目的片段产量。
Cartpieces
Totalgoods,subtotals:￥Checkout
Bought notes(bought amounts latest0)
User Comment(Total0User Comment Num)
- No comment
+86 571 56623320
SALES@SUNLONGBIOTECH.COM
+86 18668110335
