Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific recognition of Mycoplasma melitensis, no cross-reaction with the genomes of other organisms, and is not affected by Interference from other parasitic microorganisms.

2. High sensitivity, the lowest number of copies can be detected is less than 10.

3. Use hot-start Taq enzyme to inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5, with ROX reference dye to help correct sampling errors and differences between tubes.

6, with UDG enzyme and dUTP, which can reduce the contamination of residual DNA.

 

Hemobartonella< /em>) and Eperythrozoon, which were reclassified as mycoplasmas based on 16S rRNA gene sequence analysis, are found in the blood of mammals such as cats, dogs, sheep, cattle, pigs, and humans. All were discovered. There are two types of haemophilic mycoplasmas that have been found in goats and sheep: Mycoplasma ovis and Candidatus Mycoplasma hemovis. There is also a view that the two are actually two 16 species of a mycoplasma.S rRNA gene version.

 

Mycoplasma melitensis is also known as Eperythroblastosis melitensis, is the causative agent of ovine epierythrozoonosis, and its host is sheep, goats or deer. It is free in the plasma or attached to the surface of red blood cells, causing massive destruction of red blood cells. The clinical manifestations after infection can be divided into acute and chronic. The acute state is characterized by hemolytic anemia and poor exercise tolerance; the chronic state is characterized by weight loss. Decline, sluggishness; chronic state can transform into acute state under conditions such as stress, illness, pregnancy, or immunosuppression.

 

There is no suitable in vitro culture method for Mycoplasma melitensis. The detection method generally adopts Giemsa-stained blood smear method or serological method. The blood smear method has poor detection sensitivity and specificity, and cannot detect Mycoplasma ovis when the sheep is anemic. Mycoplasma ovis parasitism often occurs at a low level, blood levels fluctuate during infection, and detection requires repeated examination of blood smears. Serological methods can only prove a history of pathogen exposure and cannot reflect whether there is infection. The PCR method is more convenient and more sensitive, and the detection time is short, and the results can be obtained in just a few hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

 

This kit targets a partner of Mycoplasma sheep blood Protein gene, design specific primers, can accurately identify Mycoplasma melitensis, and have no specific reaction with other genomes. After testing, there was no cross-reaction with 6 other mycoplasmas and 12 sepsis-related bacteria that parasitize goats and sheep, as well as 7 other types of haemophilic mycoplasma.

 

This kit contains hot-start Taq enzyme, dNTP, primers, positive controls, SYBR Green I dye, ROX dye, and UDG to prevent residual DNA contamination), users only need to prepare DNA samples to use.

 

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性识别羊支原体（羊附红细胞体），与其他生物基因组无交叉反应，不受其他寄生微生物的干扰。

2， 灵敏度高，最低可检测出10个以下的拷贝数。

3， 使用热启动的Taq酶，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有ROX参比染料，帮助校正加样误差和管间差异。

6， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

嗜血支原体（Hemotropic Mycoplasma，或hemoplasma)是一类寄生于红细胞表面的细菌，曾被命名为血巴通氏体（Hemobartonella）和附红细胞体（Eperythrozoon），根据16S rRNA基因序列分析，被重新归类为支原体，在猫、犬、羊、牛、猪以及人等哺乳动物血液中都有发现。在山羊和绵羊中已发现的嗜血支原体有两种：羊支原体（Mycoplasma Ovis）和Candidatus Mycoplasma hemovis。也有观点认为这两者其实是一种支原体的两个16S rRNA基因版本。

羊支原体又名羊附红细胞体（Eperythrozoon Ovis），是羊附红细胞体病的病原体，宿主为绵羊、山羊或鹿。它游离于血浆或附着在红细胞表面，引起红细胞的大量破坏，感染后的临床表现可分为急性和慢性两种，急性状态表现为溶血性贫血、运动耐受力差；慢性状态则表现为体重下降、行动迟缓；在压力、疾病、怀孕或免疫抑制等情况下，慢性状态可转变为急性状态。

羊支原体尚无合适的体外培养方法，检测方法一般采用吉姆萨染色的血涂片法，或血清学方法。血涂片法的检测灵敏度和特异性均比较差，而且在羊已出现贫血的情况下无法检测出羊支原体。羊支原体寄生经常发生在一个较低的水平，感染过程中血液含量有波动性，对其检测需要反复的检查血涂片。血清学方法只能证明有病原暴露史，不能反映是否感染。而使用PCR法检测则更为方便，且灵敏度更高，检测时间短，仅需几个小时即可获得结果。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒针对羊血支原体的一个伴侣蛋白基因，设计特异性引物，可准确识别羊支原体,与其他基因组没有特异性反应。经检测，与寄生在山羊和绵羊的6种其他支原体和12种败血症相关细菌、以及7种其他种类的嗜血支原体，均没有交叉反应。

本试剂盒包含热启动Taq酶、dNTP（以UTP代替了TTP）、引物、阳性对照品、SYBR Green I染料、ROX染料，和用以防止残余DNA污染的UDG（尿嘧啶-N-糖苷酶），用户只需准备好DNA样品即可使用。

 

 

 

 

 

 

Cartpieces

• 
• 1

  1Delete

• 
• 6

  6Delete

• 
• q

  qDelete

• 
• D

  DDelete

• 
• +

  +Delete

• 
• 7

  7Delete

• 
• 2

  2Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 8

  8Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• D

  DDelete

• 
• �

  �Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 8

  8Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• D

  DDelete

• 
• 5

  5Delete

• 
• P

  PDelete

Totalgoods,subtotals:￥Checkout