Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma agalactiae and does not produce specific signals with the genomes of other organisms.

2. High sensitivity, the lowest detection limit is 6 genomes in the reaction solution.

3. The DNA polymerase used has the characteristics of strong anti-inhibition ability and good thermal stability; using hot start method, it can Inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5. With UDG enzyme and dUTP, it can reduce the contamination of residual DNA.

Mycoplasma agalactiae is a bacterium of the genus Mycoplasma. It is the second mycoplasma known to humans after Mycoplasma mycoides. It was first isolated in 1925 and is widely distributed around the world. It is responsible for 90% of The causative agent of infectious agalactiae syndrome in goats and nearly 100% of sheep outbreaks affects both sexes and can cause mastitis, arthritis, and keratoconjunctivitis in addition to agalactiasis. M. agalactiae has no cell wall and is therefore not affected by many common antibiotics such as penicillins or other beta-lactam antibiotics that target cell wall synthesis. The biochemical characteristics of Mycoplasma agalactiae are as follows: it lacks glucose and mannose metabolism and the ability to hydrolyze arginine and gelatin, has phosphatase activity, can reduce tetrazole under anaerobic and aerobic conditions, and has the ability to adsorb blood cells.


Mycoplasma agalactiae can be detected through in vitro culture methods and serological methods or geneticsMethods are used for detection. Mycoplasma bovis and Mycoplasma agalactiae are very similar in phenotype and genetics, and share a lot of common antigens and epitopes, so serological methods can distinguish them. It is more difficult; the two are also very similar in some metabolic pathways, which also limits the application of biochemical diagnostic methods. Serological methods are also susceptible to vaccine interference. In vitro culture methods are time-consuming and difficult to control the growth status. The PCR method for specific sequences has a short detection time, is not susceptible to interference, and has obvious advantages in sensitivity, so it has been widely used. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.


This kit selected a housekeeping gene sequence for PCR identification, and the primers were tested by BLAST Verified to specifically target Mycoplasma agalactiae and not produce specific signals with the genomes of other organisms. Specific detection using this kit includes: 34 Mycoplasma agalactiae strains, 24 other Mycoplasma strains, pathogens parasitic to small ruminants, bacteria and 6 species of eukaryotic parasites), as well as healthy ruminant animal tissues, no false negative or false positive results were found.


This kit contains hot-start DNA polymerase, dNTP, primers, probes, positive controls, ROX dye, and UDG to prevent residual DNA contamination. Users only need to prepare DNA samples and use them.

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测无乳支原体，与其他生物的基因组不产生特异性信号。

2， 灵敏度高，最低检测极限为反应液中6个基因组。

3， 使用的DNA聚合酶具有抗抑制能力强和热稳定性好的特点；采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

无乳支原体（Mycoplasma Agalactiae）是支原体属的一种细菌，是继丝状支原体之后第二个被人类所知的支原体，在1925年首次被分离出来，在全球广泛分布，是造成90％山羊和接近100％绵羊传染性无乳症综合症爆发的病原体，雌雄均可感染，除了无乳症之外，还可以引发乳腺炎、关节炎和角膜结膜炎。无乳支原体没有细胞壁，因此不受许多常见的抗生素如青霉素或靶向细胞壁合成的其他β-内酰胺抗生素的影响。无乳支原体生物化学特征如下：缺乏葡萄糖和甘露糖代谢以及对精氨酸和明胶的水解能力，有磷酸酶活性，在厌氧和有氧条件下可还原四唑，有血球吸附能力。

对无乳支原体的检测，可以通过体外培养法、血清学方法（如免疫荧光法和免疫印迹法）或遗传学方法（如PCR扩增或寡核苷酸互补配对）进行检测。牛支原体（Mycoplasma bovis）和无乳支原体在表型和遗传基因上都有很高的相似性，且拥有非常多的共同抗原和表位，因此血清学方法区别二者较为困难；二者在一些代谢通路上也很相似，因此也限制了生化诊断方法的应用。血清学方法也易受到疫苗的干扰。体外培养法耗时较长，且生长状态较难掌握。针对特异性序列的PCR法，检测时间短，不易受干扰，在灵敏度上有明显的优势，因此得到了广泛应用。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒选取了一个管家基因序列进行PCR鉴定，引物经BLAST验证为特异性靶向无乳支原体，与其他生物的基因组不产生特异性信号。使用本试剂盒进行的特异性检测包括：34个无乳支原体菌株、24种其他支原体菌株（包括牛支原体）、寄生于小型反刍类动物的病原体（包括病毒（13种）、细菌（27种）和6种真核类寄生生物）、以及健康反刍类动物组织，未发现假阴性或假阳性结果。

本试剂盒包含热启动DNA聚合酶、dNTP（以dUTP代替dTTP）、引物、探针、阳性对照品、ROX染料，和用以防止残余DNA污染的UDG（Uracil- DNA Glycosylase，尿嘧啶-DNA-糖基酶），用户只需准备好DNA样品即可使用。

 

 

 

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